Showing posts tagged with "small-molecules-and-synthetic-therapeutics"
Using a “dry” loading technique with flash chromatography typically improves compound purity and overall separation quality compared to liquid loading. The reasons for this I have prophesized previous...
Flash chromatography is a chemical separation technique used to purify chemical mixtures. Because it is a purification technology, the process is also referred to as flash purification.
Equilibrating silica flash chromatography columns is something I always do. There are chemists who see this as an unnecessary, time-and-solvent-wasting step. However, because getting consistent, predi...
This question is one that is increasing in frequency. Over the past 10 or so years reversed-phase flash chromatography use has increased dramatically. Likewise, reversed-phase preparative HPLC (RP pHP...
Many chemists today find they need to synthesize molecules at higher temperatures in order to force difficult reactions to proceed. Solvents such as DMF, DMSO, and NMP are commonly used in these react...
You have performed your synthesis and now it is time to purify the reaction mix. You have used thin-layer chromatography (TLC) and see a separation but when you try to purify with flash column chromat...
Organic and medicinal chemists frequently utilize flash chromatography to purify their reaction mixtures. Normal-phase flash chromatography is most often used but may not the best methodology, especia...
Organic chemistry can be messy with not only the desired compound created but also myriad by-products. As synthetic chemists, our goal is to make these desired compounds in sufficient yield and purity...
Knowing when it is time to replace your reversed-phase flash column is a question I am asked frequently along with…
Think of orthogonal flash chromatography as 2D-chromatography where a reaction mixture or natural product extract is purified first with one methodology or solvent gradient then re-purified with a dif...