Simplifying the Extraction of Opiates from Hydrolyzed and Non- Hydrolyzed Urine Using Automated Supported Liquid Extraction Prior to GC-MS Analysis

The opioid crisis in the United States has necessitated monitoring of these drugs in biological fluids by many state health laboratories. Urine is a common matrix for analysis, and labs require a simple yet efficient extraction process for both hydrolyzed and non-hydrolyzed samples. Here we describe a fully-automated, simplified extraction of opiates (codeine, dihydrocodeine, hydrocodone, hydromorphone, morphine, 6-MAM, oxycodone, oxymorphone) from acid-hydrolyzed urine and non-hydrolyzed urine prior to GC-MS analysis. APHL 2021 Virtual Meeting