Automated sample preparation for drugs in urine using room temperature enzymatic hydrolysis

Routine testing for drugs in urine is commonly performed by clinical, forensic, and pain management laboratories. The sample cleanliness required for analysis by LC-MS/MS can result in lengthy, labor-intensive extraction protocols. Most labs seek simplified yet reliable and robust sample preparation and analysis methods. Many laboratories turn to automation for more efficient sample preparation that reduces labor costs. Supported liquid extraction (SLE) is a simple, elegant technique that is analogous to liquid-liquid extraction, but can be easily automated. The entire sample preparation process for hydrolyzed urine was automated using the Biotage® Extrahera™ automated
extraction system. A panel of 10 conjugated metabolites of common drugs were hydrolyzed and extracted by supported liquid extraction using ISOLUTE® SLE+. Hydrolysis efficiencies were calculated to assess method functionality.  

Literature number: P228