Non-selective sample extraction approaches for broad-spectrum DoA whole blood analysis
By Biotage
Forensic, toxicology, and clinical laboratories conduct broad spectrum whole blood (WB) screening to identify potential drug exposures. Extraction of drugs of abuse (DoA) panels from WB can be challenging, especially when targeting a large panel of analytes of different classes. The protein precipitation is straightforward but cannot provide sufficient clean up for WB. Selective techniques, such as solid phase extraction (SPE), offer better sample cleanness; however, the specificity of the interaction makes it difficult to balance the extraction coverage and sample cleanliness. Using nonselective techniques can effectively remove the matrix without compromising the extraction recovery for a wide range of analytes. Here, we demonstrate an automated sample preparation workflow for the broad-spectrum DoA WB analysis using two non-selective sorbent chemistries: protein and phospholipid depletion (PLD) and supported liquid extraction (SLE).
Literature number: P266
