Perfecting Pcats – developing a more robust UHPLC-MS/MS method for plasma catecholamines

Achieving desired sensitivity of plasma catecholamines by LCMS/MS can be problematic. Low LOQs can require a highly sensitive MS instrument. Evaporation of the extracted sample can result in losses during the evaporation step, making concentration of the sample prior to analysis difficult. Removal of matrix components that interfere with analysis or suppress MS response (particularly phospholipids) is necessary for maximum sensitivity and accuracy. This study was conducted to optimize sample preparation and LCMS/MS conditions to improve recovery and reduce ion suppression for analysis of plasma catecholamines. 

Literature number: P232.V.1