Showing posts written by Austin Schlirf
Preparative HPLC and flash chromatography are two of the most utilized techniques for purifying molecules post synthesis. To monitor sample purity and elution times, detection techniques such as UV/UV...
Cl-trityl resin can be tricky to use not only from an amino acid loading perspective, but one must also consider the highly labile linkage to the solid support1. This lability is advantageous in insta...
Head-to-tail cyclization of peptides is being used in drug development as yet another way to probe how constraining peptide sequence structure in solution can improve pharmacokinetics and target speci...
The big question…what affects cyclization efficiency? This is what I have been trying to answer over the course of the last few experiments, especially when it comes to head-to-tail macrocycles. I hav...
The dynamics of solid phase peptide synthesis at the resin surface makes it particularly difficult to create head to tail macrocycles. Dimerization, steric hinderance, and C-terminal access all reduce...
Macrocyclization of peptides has arisen as a new strategy to create proteolytically stable, membrane permeable drug candidates while maintaining target specificity. To perform this cyclization, lactam...