Automated sample extraction technique for LC-MS/MS analysis of zolmitriptan in human plasma
By Biotage
Typical bioanalysis workflows for small molecules use organic solvents to precipitate proteins and extract analyte(s). Sometimes, extra clean-up, including liquid-liquid extraction (LLE) or solid phase extraction (SPE), is needed to further remove the matrix to satisfy sensitivity, accuracy, and precision requirements. However, method development for bioanalysis is rather challenging. Some operating procedures are tedious and difficult to scale up.
We provide an automated workflow using Biotage ISOLUTE® SLE+ Supported Liquid Extraction (SLE) technology to extract analyte(s) from the biomatrix. This method offers better matrix cleanup than protein precipitation, is much easier to scale up and automate than LLE and is less complicated and time-consuming than SPE.
In this study, we spiked Zolmitriptan, a selective serotonin receptor agonist, into plasma to mimic the biological samples for PK/PD studies and used it to evaluate the performance of the workflow regarding matrix effect, recovery, phospholipid removal, linearity, accuracy, and precision.
Literature number: P279
