Quantification of tramadol and its metabolites in molasses using automated EVOLUTE® EXPRESS CX SPE

Molasses is commonly used in equine feed manufacturing as a
palatability enhancer or feed binder. However, it may
occasionally be contaminated with pharmacologically active
substances. Tramadol, a synthetic opioid analgesic, along with
its primary metabolites, pose a risk of anti-doping rule
violations if inadvertently introduced via feed.
An accurate and precise quantification method for the
analysis of tramadol and its metabolites in molasses is vital to
ensure fair play within horse racing.

This poster demonstrates a robust and automated solid-phase extraction
(SPE) procedure using Biotage® EVOLUTE® Express CX (30mg)
plates. Analysis was performed using LC-MS/MS. The method
is tailored for doping control and regulatory compliance in the
equine industry.

Literature number: P288