Protein G PhyTip® column
Protein purification column
Fast, automated IgG purification with 70–90% recovery. Protein G offers high selectivity for human IgG3 and rat IgG2a.
Both protein G and protein A bind to the Fc region of IgG antibodies, with varying selectivity for different IgG subtypes. In some cases, such as human IgG3 or rat IgG2a, Protein G is the preferred choice due to its higher selectivity for these subtypes and species.
PhyTip® columns use dual flow chromatography technology, pumping samples back and forth across the packed resin to maximize binding. Efficient capture minimizes protein loss, with 70–90% recovery of lgGs, and a full 96-well plate can be purified in just 15 minutes.
The columns come with dual flow chromatography protocols, validated with real protein samples and fully transferable to any automated liquid handling system. Our buffer kits contain all the buffers needed for the purification protocol.
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Biomolecule purification with PhyTip® columns
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Product note
Protein G PhyTip® columns
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White paper
PhyTip® columns versus other common technologies: a comparative analysis of protein purification yields
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White paper
PhyTip® columns versus other common technologies: a comparative analysis of protein purification efficiency and yield consistency
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White paper
Accelerating high throughput with PhyTip® columns
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Application note
High Performance Immunoprecipitation (HPIP), direct IP method
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High Performance Immunoprecipitation (HPIP), indirect IP method
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Application note
PhyTip® columns processed on the Tecan Freedom EVO® for complete automation of high-throughput protein purification and sample preparation
Ordering information
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