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How should I make my linear gradient?
Small Molecules and Synthetic Therapeutics
This post shows the impact of gradient slope on purification results.
How important is flow rate in flash chromatography?
Small Molecules and Synthetic Therapeutics
This post discusses the impact that flow rate has on separation efficiency.
How does a flash system’s internal volume affect gradients?
Small Molecules and Synthetic Therapeutics
This post discusses the impact of a flash system's internal plumbing volume on gradient purification.
How should normal-phase gradient solvents be chosen?
Small Molecules and Synthetic Therapeutics
This post describes some methodology on selecting normal-phase solvents for flash chromatography.
When is Gradient Elution Better than Isocratic Elution?
Small Molecules and Synthetic Therapeutics
This post discusses the benefits of gradient elution vs. isocratic elution.
What is a Chromatography Gradient?
Small Molecules and Synthetic Therapeutics
This post describes what a gradient is and how one will impact chromatographic results.
What is the maximum flash column sample load volume?
Small Molecules and Synthetic Therapeutics
This post discusses the impact that injection volume has on purification results.
How does an acid pH affect reversed-phase chromatography separations?
Small Molecules and Synthetic Therapeutics
This post describes some benefits an acidified mobile phase provides.
Can reversed-phase flash column chromatography be greener?
Small Molecules and Synthetic Therapeutics
Can reversed-phase flash column chromatography be greener?
How many times can I reuse my reversed-phase flash column?
Small Molecules and Synthetic Therapeutics
This post discusses the steps that can be taken to increase reversed-phase flash column life.
