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High-throughput methods for protein purification and production are becoming more common in the biopharmaceutical and biotech industry. Advancements have occurred in high-throughput expression and clo...
Purification of immunoglobulins is essential for the development of new biopharmaceuticals and affinity chromatography is a popular method for this application. One approach to purification of immunog...
Since the therapeutic use of antibodies for cancer continues to grow since Kohler and Milstein’s work on the hybridoma technology, the need for high throughput antibody purification has become a stapl...
Reversed-phase chromatography is an essential step in any drug discovery workflow, particularly as a desalting step prior to MS/MALDI analysis. One of the first choices you must make when you design y...
As the throughput in the drug discovery process of large molecules have, in recent years, become considerably more high throughput many are looking for ways to automate their sample management.
Plasmids are one of the cornerstones of modern biology, providing the foundation for several areas of important research including DNA vaccines, mRNA vaccines, viral vectors, and monoclonal antibodies...
One of the biggest challenges with automated plasmid purification is with culture growth conditions, leading to low yielding cultures. Many times, culture growth is unpredictable and hard to perfect. ...
Successful transient transfection relies on many factors, starting with a successful plasmid prep. To ensure high transfection efficiency, resulting DNA from your plasmid preps must be transfection gr...
Gene therapy has had its share of setbacks, but finally gene therapies are proving successful at treating diseases like neuromuscular disorders, cancer and blindness. Adeno-associated virus (AAV) has ...
Now that you have your purified plasmid, you will need to get an idea of its quality. Transfection efficiency is highly influenced by the quality of the plasmid, so it’s important to get a full pictur...