How to check the quality of plasmid DNA
Now that you have your purified plasmid, you will need to get an idea of its quality. Transfection efficiency is highly influenced by the quality of the plasmid, so it’s important to get a full pictur...
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Now that you have your purified plasmid, you will need to get an idea of its quality. Transfection efficiency is highly influenced by the quality of the plasmid, so it’s important to get a full pictur...
Plasmids are an indispensable tool that are used to clone, transfer, and manipulate genes. These small, circular fragments of DNA are typically found in bacteria but are also present in some archaea a...
When in the lab I always try to streamline and improve workflows. One of the biggest bottlenecks in the lab I’ve experienced and maybe you have too, is the drying of sample extracts.
Solid phase extraction (SPE) is a powerful sample preparation tool that makes it possible to extract semi-volatile organic compounds with varying physical and chemical properties. When used properly, ...
Every step within a solid phase extraction (SPE) procedure is imperative, but one of the most important steps that must be done properly is the air-dry step.
If you’re like me, you start the new year off with a list of resolutions for the coming months – resolutions to be more fit or to secure a promotion at work, or to reduce your carbon footprint.
Bisphenol A (BPA) is one of the most widely produced chemicals in the world – approximately 4 million metric tons annually. In recent years, BPA has received a lot of negative attention. In fact, I ca...
In a never-ending list of chemical pollutants, a compound that is gaining a lot of attention is 1,4-dioxane. In fact, New Jersey just became the first state to set regulations on the quantity of 1,4-d...
As a chemist, I’ve constantly stressed the importance of proper sample preparation. Whether I’m diluting, digesting, preconcentrating, extracting, or performing a combination of these, sample preparat...
In the world of solid phase extraction (SPE), the list of media that is available seems to be ever-growing. From polymeric stationary phases, to silica-based media, and even molecularly imprinted poly...