Automation of MiniPrep in a Drug Discovery Setting
As the throughput in the drug discovery process of large molecules have, in recent years, become considerably more high throughput many are looking for ways to automate their sample management.
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As the throughput in the drug discovery process of large molecules have, in recent years, become considerably more high throughput many are looking for ways to automate their sample management.
Plasmids are one of the cornerstones of modern biology, providing the foundation for several areas of important research including DNA vaccines, mRNA vaccines, viral vectors, and monoclonal antibodies...
One of the biggest challenges with automated plasmid purification is with culture growth conditions, leading to low yielding cultures. Many times, culture growth is unpredictable and hard to perfect. ...
Successful transient transfection relies on many factors, starting with a successful plasmid prep. To ensure high transfection efficiency, resulting DNA from your plasmid preps must be transfection gr...
Gene therapy has had its share of setbacks, but finally gene therapies are proving successful at treating diseases like neuromuscular disorders, cancer and blindness. Adeno-associated virus (AAV) has ...
Now that you have your purified plasmid, you will need to get an idea of its quality. Transfection efficiency is highly influenced by the quality of the plasmid, so it’s important to get a full pictur...
Plasmids are an indispensable tool that are used to clone, transfer, and manipulate genes. These small, circular fragments of DNA are typically found in bacteria but are also present in some archaea a...
When in the lab I always try to streamline and improve workflows. One of the biggest bottlenecks in the lab I’ve experienced and maybe you have too, is the drying of sample extracts.
Solid phase extraction (SPE) is a powerful sample preparation tool that makes it possible to extract semi-volatile organic compounds with varying physical and chemical properties. When used properly, ...
Every step within a solid phase extraction (SPE) procedure is imperative, but one of the most important steps that must be done properly is the air-dry step.