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Recently, one of our readers wrote and asked how to determine solvent strength in normal-phase flash chromatography. This is an excellent question because solvent strength is one of several factors im...
In my previous post, I talked about the "Chemistry Behind Normal-phase Flash Chromatography", the most common form of liquid-solid chromatography. In this post, I focus on reversed-phase flash chromat...
For most organic and medicinal chemists flash chromatography is just another step in the synthesis work flow - react, analyze, purify, react, analyze, purify... until the final product is made. The de...
Reversed-phase flash chromatography usage is increasing rapidly. In fact, over the past 10 or so years, reversed-phase flash chromatography use has increased a dramatic 650%! This is amazing growth de...
Flash chromatography competing with prep-HPLC? Why and when would flash outperform or even be equal to prep HPLC? Well, it depends on your purification needs and goals.
Getting the most benefit from your crude sample purification with column chromatography or flash chromatography involves optimizing many variables.
Synthetic chemists continually seek ways to create novel compounds. Along the way they evaluate reagents, solvents, and reaction conditions giving rise to various reaction products and by-products.
In this article I discuss the optimization of solvent ratios to generate ideal Rf (retention factor) values on TLC plates. Then I show how maximizing efficiency of flash chromatography achieves higher...
Underloading your SLE+ samples can result in better partitioning and cleaner samples. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on...
One of the hottest, yet most neglected topics in sample preparation is evaporation. Most chromatographic methods require some form of sample cleanup prior to injection into a HPLC or GC system to prev...