Which loading method should I use for purification by flash chromatography?
Getting the most benefit from your crude sample purification with column chromatography or flash chromatography involves optimizing many variables.
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How should I purify a complex, polar, amide reaction mixture?
Synthetic chemists continually seek ways to create novel compounds. Along the way they evaluate reagents, solvents, and reaction conditions giving rise to various reaction products and by-products.
How to Optimize TLC to Enhance Purification by Flash Chromatography
In this article I discuss the optimization of solvent ratios to generate ideal Rf (retention factor) values on TLC plates. Then I show how maximizing efficiency of flash chromatography achieves higher...
Why should I underload SLE+ plate or cartridge?
Underloading your SLE+ samples can result in better partitioning and cleaner samples. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on...
Simplifying solvent evaporation for sample prep with next generation systems
One of the hottest, yet most neglected topics in sample preparation is evaporation. Most chromatographic methods require some form of sample cleanup prior to injection into a HPLC or GC system to prev...
Why and when is florisil cleanup necessary with sample extracts?
No matter what your sample will be tested for, if you are extracting with liquid-liquid extraction (LLE), solid phase extraction (SPE), accelerated solvent extraction (ASE) or microwave, your sample's...
What are peak asymmetry requirements for PFAS using EPA Method 537.1?
In the United States, the Environmental Protection Agency (EPA) establishes performance criteria in which laboratories must demonstrate their technical capability to produce results that are accurate ...
High-throughput Protein Purification Strategies
Antibodies are indispensable tools that are used in a variety of research applications, diagnostics, and therapeutics. Often, these applications require purified antibodies which means that the antibo...
How St. John's University Accelerated their Cancer Research with the Biotage Peptide Workflow Solution
"How can I take a two hour purification using old-school chromatography, and shorten it?" was the question Dr. Aaron Muth at St. John's University in New York asked himself. "By using the Biotage Isol...
What do you do when your peptide synthesis fails?
It never fails. A sequence looks relatively straightforward on paper and it's of moderate length so you start synthesizing without too much thought into the protocol. Your synthesis is finished and yo...