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Synthetic chemists continually seek ways to create novel compounds. Along the way they evaluate reagents, solvents, and reaction conditions giving rise to various reaction products and by-products.
In this article I discuss the optimization of solvent ratios to generate ideal Rf (retention factor) values on TLC plates. Then I show how maximizing efficiency of flash chromatography achieves higher...
Underloading your SLE+ samples can result in better partitioning and cleaner samples. Supported liquid extraction (SLE) is a simple clean-up technique similar to liquid-liquid extraction (LLE), but on...
One of the hottest, yet most neglected topics in sample preparation is evaporation. Most chromatographic methods require some form of sample cleanup prior to injection into a HPLC or GC system to prev...
No matter what your sample will be tested for, if you are extracting with liquid-liquid extraction (LLE), solid phase extraction (SPE), accelerated solvent extraction (ASE) or microwave, your sample's...
In the United States, the Environmental Protection Agency (EPA) establishes performance criteria in which laboratories must demonstrate their technical capability to produce results that are accurate ...
Antibodies are indispensable tools that are used in a variety of research applications, diagnostics, and therapeutics. Often, these applications require purified antibodies which means that the antibo...
"How can I take a two hour purification using old-school chromatography, and shorten it?" was the question Dr. Aaron Muth at St. John's University in New York asked himself. "By using the Biotage Isol...
It never fails. A sequence looks relatively straightforward on paper and it's of moderate length so you start synthesizing without too much thought into the protocol. Your synthesis is finished and yo...
Our seasoned peptide chemist Dr. Elizabeth Denton has tried it all when it comes to peptides. In this short video she shows that using DMSO (dimethyl sulfoxide) in a peptide synthesis workflow is perf...