CLOSE ×
    New Biotage® PhyPrep Maxi Mega Giga
    Endotoxin free Plasmid purification, automated.

    Antibody Purification

    Automated Antibody Purification by PhyTip® Columns

    Small-scale antibody purification is fundamental in biologics research and development.  Our unique PhyTip columns enables automated antibody purification with high recoveries, while being gentle on the protein, yielding consistent results. Read more about our technology here. PhyTip columns are available packed with carefully selected high quality affinity resins for effective sample processing and purification protocols that have been validated with real protein samples.

    PHR-96-000-10ab
    biomark002.397 - Antibody Structure and Fragments

    Affinity Purification with PhyTip Columns

    Immunoglobins consist of four polypeptide chains – two identical heavy chains and two identical light chains. Both the heavy and light chains consist of different regions called the constant (C) and the variable (V) domains. Structural differences between the heavy chains allows immunoglobins to be divided into five different sub classes - IgG, IgM, IgA, IgE and IgD. Furthermore, immunoglobin light chains exist in two different types, which are referred to as lambda (λ) and kappa (κ). Modern cloning technologies also enable expression of recombinant antibody fragments, such as Fab fragments, Fv fragments and Fc fragments.

    Biotage offers PhyTip columns packed with different affinity medias that binds selectively to various parts of the antibody structure for purification IgG antibodies as well as other subtypes and fragments.

    Product Groups

    biomark002.399 -Protein G
    Protein A PhyTip® Columns

    Purification of IgG antibodies and fragments that include the Fc region by binding to the Fc region.

    biomark002.400 - Proplus
    Protein G PhyTip® Columns

    Purification of IgG antibodies and fragments that include the Fc region by binding to the Fc region with selectivity to certain subtypes.

    biomark002.400 - Proplus
    ProPlus PhyTip® Columns

    Purification of IgG antibodies and fragments that include the Fc region by binding to the Fc region by oriented binding by MabSelect™ SuRe™, Cytiva.

    biomark002.401 - Captue Select Lambda-1
    CaptureSelect™ LC-Lambda (Hu) PhyTip® Columns

    Purification of human IgGs by binding to the lambda light chain.

    biomark002.402 - Capture Select KappaXL
    CaptureSelect™ KappaXL PhyTip® Columns

    Purification of IgA, IgM, IgD, IgE and their Fab and Fab2 fragments by binding to the kappa light chain.

    biomark002.403 - Capture Selekt FcXL
    CaptureSelect™ FcXL PhyTip® Columns

    Purification of human IgG subclasses by binding to the CH3 domain.

    biomark002.399 -Protein G
    CaptureSelect™ IgG-Fc (ms) PhyTip® Columns

    Purification of IgG in various species by binding to the Fc region.

    IgM
    POROS CaptureSelect™ IgM PhyTip® Columns

    Purification of IgM by binding to the Fc region.

    News from our blog
    Resources

    We offer PhyTip columns for all major liquid handling systems.

    Other Blocks in the workflow

    PhyTip® columns for automated purification of tagged proteins.

    Protein purification by ion exchange PhyTip columns.

    Automated high throughput desalting and buffer exchange.