Antibody Purification

Automated Antibody Purification by PhyTip® Columns

Small-scale antibody purification is fundamental in biologics research and development. Our unique PhyTip columns enables automated antibody purification with high recoveries, while being gentle on the protein, yielding consistent results. Read more about our technology here. PhyTip columns are available packed with carefully selected high quality affinity resins for effective sample processing and purification protocols that have been validated with real protein samples.

PHR-96-000-10ab

biomark002.397 - Antibody Structure and Fragments

Affinity Purification with PhyTip Columns

Immunoglobins consist of four polypeptide chains – two identical heavy chains and two identical light chains. Both the heavy and light chains consist of different regions called the constant (C) and the variable (V) domains. Structural differences between the heavy chains allows immunoglobins to be divided into five different sub classes - IgG, IgM, IgA, IgE and IgD. Furthermore, immunoglobin light chains exist in two different types, which are referred to as lambda (λ) and kappa (κ). Modern cloning technologies also enable expression of recombinant antibody fragments, such as Fab fragments, Fv fragments and Fc fragments.

Biotage offers PhyTip columns packed with different affinity medias that binds selectively to various parts of the antibody structure for purification IgG antibodies as well as other subtypes and fragments.

Product Groups

biomark002.399 -Protein G

Protein A PhyTip® Columns

Purification of IgG antibodies and fragments that include the Fc region by binding to the Fc region.

biomark002.400 - Proplus

Protein G PhyTip® Columns

Purification of IgG antibodies and fragments that include the Fc region by binding to the Fc region with selectivity to certain subtypes.

biomark002.400 - Proplus

ProPlus PhyTip® Columns

Purification of IgG antibodies and fragments that include the Fc region by binding to the Fc region by oriented binding by MabSelect™ SuRe™, Cytiva.

biomark002.402 - Capture Select KappaXL

CaptureSelect™ KappaXL PhyTip® Columns

Purification of IgA, IgM, IgD, IgE and their Fab and Fab2 fragments by binding to the kappa light chain.

biomark002.403 - Capture Selekt FcXL

CaptureSelect™ FcXL PhyTip® Columns

Purification of human IgG subclasses by binding to the CH3 domain.

biomark002.399 -Protein G

CaptureSelect™ IgG-Fc (ms) PhyTip® Columns

Purification of IgG in various species by binding to the Fc region.

IgM

POROS CaptureSelect™ IgM PhyTip® Columns

Purification of IgM by binding to the Fc region.

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Overview of Compatible Liquid Handling Systems

We offer PhyTip columns for all major liquid handling systems.

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Other Blocks in the workflow

PhyTip® columns for automated purification of tagged proteins.

Purification of Tagged Protein

Protein purification by ion exchange PhyTip columns.

Ion Exchange

Automated high throughput desalting and buffer exchange.

Gel Filtration PhyTip Columns

About Biotage

Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in drug discovery and development and diagnostics, analytical testing and water and environmental testing.

Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.

Our customers span a broad range of market segments including pharmaceutical, biotech, diagnostic, contract research and contract manufacturers as well as clinical, forensic and academic laboratories in addition to organizations focused on food safety, clean water and environmental sustainability.

Biotage is headquartered in Uppsala in Sweden and employs appr. 700 people worldwide. The Group had sales of 1,566 MSEK in 2022 and our products are sold in more than 80 countries. Biotage’s share (BIOT) is listed in the Large Cap segment on the NASDAQ Stockholm.

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Antibody purification application page