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PhyTip® columns

High-recovery protein and biomolecule purification

Biotage PhyTip® column technology is designed for small-scale, high-throughput purification of biomolecules such as antibodies, recombinant proteins, plasmids, and viral vectors. It enables rapid processing of hundreds to thousands of potential leads, ready for cell-based assays without the need for scale-up.

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Up to 90% sample recovery with dual flow technology

PhyTip® columns use dual flow chromatography, where samples are pumped back and forth across the packed resin bed inside the tip. This repeated cycle with controlled flow gives biomolecules enough time to interact with the available binding sites, delivering high sample recovery.

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Compatible with all leading liquid handling robots

PhyTip® Columns are compatible with our semi-automated ME system as well as with all major liquid handling robots, including Hamilton, Tecan, and Beckman. Ready to use straight out of the box, they enable purification of 96 samples in as little as 15 minutes, depending on volume. Methods are simple to develop, and validated protocols are available for many platforms.

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PhyTip columns for protein a purification

A smarter choice for efficient protein purification

PhyTip® columns automate purification, maximize binding efficiency, and reduce processing time. Unlike other technologies, they maintain high yields even with small resin beds. This paper compares PhyTip® columns with filter plates, spin columns, and magnetic beads, showing protein yields across platforms.

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Plasmid purification

A reliable solution for plasmid purification, delivering consistent yields of up to 15 μg of plasmid DNA per 1 mL culture pellet. The purified DNA is of high quality and suitable for transfection, transformation, sequencing, PCR, and cloning.

MiniPrep PhyTip® column

Antibody purification

Automated antibody purification with high recovery while remaining gentle on the protein, delivering consistent results. Each column is packed with carefully selected, high-quality affinity resins and supported by validated purification protocols developed with real protein samples.

Protein A PhyTip® columns Protein G PhyTip® columns

AAV purification

Automated purification of adeno-associated viruses using CaptureSelect™ AAVX resin for high selectivity, 60-80% recovery, and fast, consistent results.

AAV PhyTip® column

Purification of tagged proteins

Automated purification of his-, GST-, biotain-tagged recombinant proteins with high recovery while remaining gentle on the protein, delivering consistent, reliable results.

IMAC PhyTip® columns Streptavidin PhyTip® columns

Reversed phase chromatography

Automated desalting or cleanup of protein and peptide samples before mass spectrometry analysis.

C4 300 Å PhyTip® columns C18 90 Å PhyTip® columns

Ion-exchange chromatography

Efficient purification of small protein samples using ion exchange chromatography, either as a standalone step or as an orthogonal polishing step after affinity chromatography, enables the production of highly pure final protein products.

Strong Anion Exchange PhyTip® column Weak Cation Exchange PhyTip® column

Gel filtration

Automated solution for desalting protein samples and performing buffer exchange, packed with size-exclusion chromatography resin, providing >80% protein yield and >95% salt removal.

Gel Filtration PhyTip® columns

Related products

C18 300 Å PhyTip® column | Reversed phase column

Automated desalting and cleanup of protein and peptide samples prior to mass spectrometry analysis. Fast, consistent, and scalable results.

Glutathione PhyTip® column | GST-tagged proteins automated purification

Automated affinity purification of GST-tagged proteins using Glutathione PhyTip® columns with 60–80% recovery and up to 90% purity.

ProPlus PhyTip® column | Automated IgG antibody purification

ProPlus and ProPlus LX PhyTip columns is packed with MabSelect™ SuRe™ and MabSelect™ SuRe™ LX affinity media from Cytiva for IgG purification.

CaptureSelect™ PhyTip columns for biomolcule purification

CaptureSelect PhyTip columns for biomolecule purification.