Showing posts written by Bob Bickler
Are you observing more chromatographic peaks than you expect compared to TLC or other assessment data? Well, it could be that your method is separating some isomers or, it could be that there is an ac...
Dry loading crude samples for flash purification typically works better than liquid loading, especially for challenging purifications. In this post, I discuss how the ratio of crude sample to dry load...
How does flow rate impact my flash column chromatography separation? This is the kind of question I frequently get. After all, we all know that flow rates that are too high or too low can result in ba...
In this post I will delve into six key factors that impact your purification success in flash column chromatography.
Plate count is a theoretical number describing the separation efficiency of a chromatography column. In short, it is a measure an eluting compound's bandwidth at the time it elutes from a column, Equa...
In all my years of working with medicinal and organic chemists, I have found that choosing how many grams of silica to use for purification by flash chromatography is something frequently guessed at. ...
Yes, the title is a bit salacious but it got your attention, didn’t it? I believe this is a topic worthy of discussion as it relates to flash chromatography for purification because many chemists beli...
In a previous post I talked about column size, specifically long-thin versus short-fat and the impact of the cartridge’s dimensions on purification performance. With that comparison I showed that in p...
This is a question being asked of my colleagues and me more and more frequently, especially in pharma accounts. Why? Well, you are familiar with the adage – Time is Money, right. Well this really appl...
Most flash column manufacturers now offer “high performance” flash chromatography columns with the promise of higher loading, increased purity, and even reduced solvent consumption. Working for Biotag...