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Before we get into our extractions, we need to set up our LC/MS to detect our analytes of interest. The LC will provide the chromatography - or separation of our analytes - and the MS will support det...
Evaporative crosstalk is contamination that occurs during evaporation of the elution solvent after SLE or SPE extraction. Volatile analytes evaporate, then redistribute in adjacent wells in a 96-well ...
You've just decided to develop a solid phase extraction (SPE) method for a new assay in your lab. However, there are so many decisions to be made! Which company to choose? Which product to use? Polyme...
We’ve all been there. We prepare a batch of samples to analyze, prime our systems, warm up the detector, and within 2-3 injections we receive an error message stating that an integral component has fa...
Conversations are routinely held regarding handling hydrophobic peptides, but hydrophilic peptides offer their own challenges when it comes to purification.
Every now and again I hear the question “which solvent do you recommend for my solid phase peptide synthesis?” Historically, dichloromethane (DCM) was used as a solvent for solid phase synthesis as th...
Sometimes the most interesting peptides are also the absolute worst to work with. Whether it's synthetic difficulty, or solubility issues, or purification difficulty, or worst of them - all of the abo...
This is a question that I am often asked by very smart people that just haven’t had time to learn the process. An optimized sample preparation method is critical for an accurate, specific, robust clin...
Most clinical chemists have developed a blood, serum or plasma assay using a protein crash because it is inexpensive and generally removes proteins that interfere with detection or the analysis in som...
When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques.