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Every now and again I hear the question “which solvent do you recommend for my solid phase peptide synthesis?” Historically, dichloromethane (DCM) was used as a solvent for solid phase synthesis as th...
Sometimes the most interesting peptides are also the absolute worst to work with. Whether it's synthetic difficulty, or solubility issues, or purification difficulty, or worst of them - all of the abo...
This is a question that I am often asked by very smart people that just haven’t had time to learn the process. An optimized sample preparation method is critical for an accurate, specific, robust clin...
Most clinical chemists have developed a blood, serum or plasma assay using a protein crash because it is inexpensive and generally removes proteins that interfere with detection or the analysis in som...
When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques.
Highly sensitive techniques such as LC-MS/MS are enabling analytical laboratories to generate more data on more analytes in more samples, in less time, at a lower cost, and using smaller sample volume...
Solid phase extraction (SPE) is frequently used for sample clean up, trace enrichment (pre-concentration of the sample), or a combination of both prior to analysis. But can solid phase extraction be a...
A prime objective of bioanalytical sample preparation is to achieve high analyte recovery in low elution volumes, with rapid delivery of clean extracts that support robust high sensitivity analysis to...
Urine samples are composed of many interferents which can cause ion-suppression or ion-enhancement when analyzing by mass spec. Build up of these matrix components over multiple sample injections can ...
What's the process behind tuning our analytes to the mass spectrometer’s optics? And why is that so important?