How do I develop a sample prep method?
This is a question that I am often asked by very smart people that just haven’t had time to learn the process. An optimized sample preparation method is critical for an accurate, specific, robust clin...
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Protein precipitation vs. traditional protein crash: what's best?
Most clinical chemists have developed a blood, serum or plasma assay using a protein crash because it is inexpensive and generally removes proteins that interfere with detection or the analysis in som...
When should I choose SPE instead of SLE?
When would I choose SLE? When would I choose SPE? We all have faced those questions. Let's have a look at the two sample preparation techniques.
How to meet demands for detection limits with the right sample prep method
Highly sensitive techniques such as LC-MS/MS are enabling analytical laboratories to generate more data on more analytes in more samples, in less time, at a lower cost, and using smaller sample volume...
The revolution of microelution in solid phase extraction
Solid phase extraction (SPE) is frequently used for sample clean up, trace enrichment (pre-concentration of the sample), or a combination of both prior to analysis. But can solid phase extraction be a...
How to achieve faster sample prep, cleaner extracts, and instant sensitivity gains
A prime objective of bioanalytical sample preparation is to achieve high analyte recovery in low elution volumes, with rapid delivery of clean extracts that support robust high sensitivity analysis to...
Dilute & shoot ... yourself in the foot
Urine samples are composed of many interferents which can cause ion-suppression or ion-enhancement when analyzing by mass spec. Build up of these matrix components over multiple sample injections can ...
Putting it together: why should I tune my mass spectrometer?
What's the process behind tuning our analytes to the mass spectrometer’s optics? And why is that so important?
Troubleshooting Loss of Signal: Where did my peaks go?
Let’s take a look at one of the more irritating issues in LC/MS method development: signal loss. I’m not talking about signal attenuation, which can be much more convoluted to diagnose; but rather a c...
Why just measure Vitamin D when you can monitor Vitamins A, E and K as well?
A new method has been developed for the combined analysis of Vitamin A, Vitamin D, Vitamin E and Vitamin K from serum using LC-MS/MS. The method uses supported liquid extraction (SLE) with a novel sam...