How to choose between SPE and SLE?
Confused about how to choose the right sample preparation technique for your compound? Don't know if Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE) would be best for your sample pre...
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What is the Role of LogP in Sample Prep Methods?
Understanding the chemical properties of the compounds in your sample matrix, both the ones you want to detect and the ones you want to eliminate, is necessary for successful method development. Is th...
Urine hydrolysis: how did I choose which enzyme to use?
Urine hydrolysis is a part of sample preparation that we do to eliminate glucuronides before urine analysis. So first, what are glucuronides? And why do we want to get rid of them? In this post, I’ll ...
In-well hydrolysis plates: What are they and do they really work?
One of the key features of the so called "in-well hydrolysis" is that it can be a time saver for high throughput labs, which makes it look very appealing. In this blog post, I will be discussing some ...
Easy protocol to develop your Supported Liquid Extraction method
Taking the time to develop a Supported Liquid Extraction (SLE) method using easy steps can save you hours or days of headaches down the road as well as giving you the peace of mind that you’re getting...
What’s the Best Way to do Supported Liquid Extraction?
Supported Liquid Extraction (SLE) is a sample preparation technique that has been in use for over ten years now, but many analytical chemists don’t know about, or understand the best way to do an SLE ...
How to determine recovery and matrix effects for your analytical assay
For many of us developing an analytical assay requires numerous experiments in addition to lots of data review, and yet despite the feeling of confidence in our success, how reliable is our assay real...
Why is pH adjustment important for sample prep methods?
How the acid dissociation constant (pKa) affects sample preparation method development? Knowing and understanding the pKa of your compounds tells you if the compound can be ionized, and under what con...
When should I choose a mixed-mode SPE?
Mixed mode Solid Phase Extraction (SPE) phases have become very popular for sample clean-up prior to analysis using mass spectrometry. Having the capability to retain compounds by two modes of interac...
When should I choose weak ion exchange SPE phases?
What is a weak ion exchange SPE phase? What is their charge state and how they behave in function of pH conditions? When to choose them? This post will describe Biotage mixed mode weak ion exchange SP...