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Taking the time to develop a Supported Liquid Extraction (SLE) method using easy steps can save you hours or days of headaches down the road as well as giving you the peace of mind that you’re getting...
Supported Liquid Extraction (SLE) is a sample preparation technique that has been in use for over ten years now, but many analytical chemists don’t know about, or understand the best way to do an SLE ...
For many of us developing an analytical assay requires numerous experiments in addition to lots of data review, and yet despite the feeling of confidence in our success, how reliable is our assay real...
How the acid dissociation constant (pKa) affects sample preparation method development? Knowing and understanding the pKa of your compounds tells you if the compound can be ionized, and under what con...
Mixed mode Solid Phase Extraction (SPE) phases have become very popular for sample clean-up prior to analysis using mass spectrometry. Having the capability to retain compounds by two modes of interac...
What is a weak ion exchange SPE phase? What is their charge state and how they behave in function of pH conditions? When to choose them? This post will describe Biotage mixed mode weak ion exchange SP...
When it comes to sample cleanup the question occasionally comes up asking what technique is best for my application: Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE)? Well, what’s rig...
There is often confusion as to how to choose the proper SPE ion exchange media. How do you determine if you should be looking at anion or cation, weak or strong? In this post, I will give some simple ...
When running through the exhaustive process of method development, most of us put the majority of our focus on validation and how to complete our crazy validation checklists. Throughout this process, ...
LC/MS/MS seems to be the gold standard in sample analysis nowadays. However, some labs are still analyzing samples using GC/MS. The majority of published peer-reviewed articles are for LC/MS/MS, so th...