When should I choose a mixed-mode SPE?
Mixed mode Solid Phase Extraction (SPE) phases have become very popular for sample clean-up prior to analysis using mass spectrometry. Having the capability to retain compounds by two modes of interac...
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When should I choose weak ion exchange SPE phases?
What is a weak ion exchange SPE phase? What is their charge state and how they behave in function of pH conditions? When to choose them? This post will describe Biotage mixed mode weak ion exchange SP...
When should I choose SLE or SPE for sample cleanup?
When it comes to sample cleanup the question occasionally comes up asking what technique is best for my application: Solid Phase Extraction (SPE) or Supported Liquid Extraction (SLE)? Well, what’s rig...
Choosing the best Ion Exchange Mode for Solid Phase Extraction
There is often confusion as to how to choose the proper SPE ion exchange media. How do you determine if you should be looking at anion or cation, weak or strong? In this post, I will give some simple ...
How to Monitor and Prevent Sample Carryover during Method Development
When running through the exhaustive process of method development, most of us put the majority of our focus on validation and how to complete our crazy validation checklists. Throughout this process, ...
How does my sample prep change between GC/MS and LC/MS/MS?
LC/MS/MS seems to be the gold standard in sample analysis nowadays. However, some labs are still analyzing samples using GC/MS. The majority of published peer-reviewed articles are for LC/MS/MS, so th...
Determining the Optimal Sample Size for your Sorbent Bed Mass
In this blog post, we will discuss how to determine the optimal sample volume for a sorbent bed mass. We have developed an adequate solid phase extraction method for our laboratory. However, we're sta...
Vacuum or positive pressure for processing samples: what's best?
Solid-phase extractions (SPE) can be a long and sometimes complicated process. So, we want to make sure that everything works the first time we extract. In this blog post, I will be discussing some of...
Techniques for disrupting protein binding in sample preparation
If you are frustrated with low recoveries caused by protein binding during Supported Liquid Extraction (SLE) and Solid Phase Extraction (SPE), this blog post will give you a few tips. Proper sample pr...
How to extract and analyze for ever-present contaminants of interest?
It happens to all of us. We're getting a new method developed and validated and then it comes time to run our negative urines. And everything comes up as positive! There are peaks for our analytes of ...