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As a peptide chemist, I was trained to purify my peptides with reversed-phase HPLC, just as many a peptide chemist before me. Despite the hundreds of hours I’ve logged in front of an HPLC, injecting s...
There are several strategies often employed to improve peptide purity achieved using reversed phase HPLC. These strategies can include, changing column length, particle size, particle functionality (C...
One common technique in HPLC for improving difficult peptide separations is to extend the column length, a topic I explored for flash chromatography in a previous post. However, alternative purificati...
Peptides, while exhibiting potential for unique specificity and affinity, still suffer from stability issues when introduced to a biological system. One strategy to overcome these stability issues is ...
Peptides, by nature, are composed of amino acids with potentially ionizable chemical moieties. The ionization state of any of these moieties can significantly impact the peptide’s chromatographic beha...
As the complexity of peptides continues to grow, so does the use of amino acids with side chain protecting groups that can be selectively removed, leaving the peptide on resin and the remaining side c...
Orthogonal amino acid protecting groups effectively expand the chemical tool kit available to peptide chemists allowing for synthesis of much more complex molecules. Often times, orthogonal protecting...
Disulphide rich peptides are being identified in species of both plants and animals at increasing rates. As new molecules are discovered and disulphide bonding patterns characterized, the need for sim...
In a previous post, I did some work evaluating the efficiency of alloc removal with tetrakis palladium using microwave assistance and atmospheric conditions, which worked beautifully.
Hydrocarbon stapling as a strategy to stabilize secondary structures of peptides, while introduced by Miller, Blackwell and Grubbs in the mid 1990s, really grew to the forefront with seminal work by S...