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In my role as a peptide application scientist, I have had the pleasure of working with many groups that are venturing into the world of peptides for the first time. Although it seems rather straightfo...
Would you ever consider an alternative to reversed- phase HPLC to purify your synthetic peptides? It seems like a silly question, right. And like many of you, I literally laughed at my Product Manager...
More and more we are seeing groups that would historically undertake only traditional organic chemistry or possibly biochemistry/biology, incorporate peptides into their research programs. While this ...
There are many techniques available to analyse and identify synthetic compounds that we are taught in the first few years of our chemistry education. While tools like NMR spectroscopy, IR spectroscopy...
"50% of my time as a Ph.D. was spend baby-sitting columns," stated Dr. László Kürti during our interview in February. Keep reading to find out more about how his team was able to increase their produc...
Your choice of sample loading technique can, and likely will, affect the separation and purity of your targeted compound. While liquid loading is easy and often fit for purpose, it can provide some is...
Purifying polar organic compounds can be very challenging. In a previous post I have discussed using reversed-phase flash chromatography to retain and purify ionizable and ionic compounds. My colleagu...
Usually, a 2-solvent or binary gradient will separate your desired compound from the by-products and impurities. Sometimes though, you can encounter a mixture in which some compounds co-elute and are ...
Flash purification is a preparative liquid chromatography technique. As such, it incorporates the same types of components as preparative high-pressure liquid chromatography (pHPLC) – pump, gradient m...
The challenges organic, medicinal, and natural product chemists face are many: from designing reactions, to optimizing synthesis, work-up / extraction, and purification / isolation of the desired comp...