| 2023 |
Structure elucidation of a human melanocortin-4 receptor specific orthosteric nanobody agonist
Research Square Oct 2023
Product used: IMAC PhyTip® Columns |
Discovery of neutralizing SARS-CoV-2 antibodies enriched in a unique antigen specific B cell cluster
PLOS ONE Sep 2023
Product used: Protein A PhyTip® Columns |
Examining SRP pathway function in mRNA localization to the endoplasmic reticulum
RNA, Jul 2023
Product used: C18 PhyTip Columns |
Detection of synthetic analogues of insulin-like growth factor 1 in different biological fluids by liquid chromatography quadrupole time-of-flight mass spectrometry: comparison of different immunoaffinity protocols
Analytical and Bioanalytical Chemistry, Aug 2023
Product used: Protein A PhyTip® Columns |
2022 White Paper on Recent Issues in Bioanalysis: Enzyme Assay Validation, BAV for Primary End Points, Vaccine Functional Assays, Cytometry in Tissue, LBA in Rare Matrices, Complex NAb Assays, Spectral Cytometry, Endogenous Analytes, Extracellular Vesicles Part 2 – Recommendations on Biomarkers/CDx, Flow Cytometry, Ligand-Binding Assays Development & Validation; Emerging Technologies; Critical Reagents Deep Characterization
Bioanalysis, Aug 2023
Product used: Streptavidin PhyTip Columns |
High-Throughput and Format-Agnostic Mispairing Assay for Multispecific Antibodies Using Intact Mass Spectrometry
Analytical Chemistry, Jun 2023
Product used: ProPlus PhyTip® Columns |
N-glycans from serum IgG and total serum glycoproteins specific for endometriosis
Nature Scientific Reports, Jun 2023
Product used: Protein G PhyTip® Columns |
Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis
Communications Biology, May 2023
Product used: IMAC PhyTip® Columns
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Efficient evolution of human antibodies from general protein language models
Nature Biotechnology, April 2023
Product used: ProPlus PhyTip® Columns |
Discovery of Nanomolar DCAF1 Small Molecule Ligands
Journal of Medicinal Chemistry, March 2023
Product used: IMAC PhyTip® Columns |
Streamlined process for automated plasmid purification reduces labour time and increases titres for AAV production
KTH - Royal Institute of Technology, March 2023
Product used: Biotage® PhyPrep System
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Neoantigen-targeted CD8+ T cell responses with PD-1 blockade therapy
Nature, March 2023
Product used: IMAC PhyTip® Columns |
An Integrated In Vivo/In Vitro Protein Production Platform for Site-Specific Antibody Drug Conjugates
Bioengineering, February 2023
Product used: ProPlus PhyTip® Columns, Protein A PhyTip® Columns
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An automated, low volume, and high-throughput analytical platform for aggregate quantitation from cell culture media
Journal of Chromatography A, February 2023
Product used: ProPlus PhyTip® Columns
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The post-translational modification profile 1 of TAR DNA-Binding Protein (TDP-43) in platelets of patients with Alzheimer's disease: An exploratory study for blood-based biomarker development
bioRxiv, January 2023
Product used: Protein A PhyTip® Columns
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Quantitative profiling of N-glycosylation of SARS-CoV-2 spike protein variants
Glycobiology, February 2023
Product used: Normal Phase PhyTip® Columns
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Stability and Requirement for Thiamin in a Cell Culture Feed Used to Produce New Biological Entities
Cells, January 2023
Product used: Protein A PhyTip® Columns
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| 2022 |
Targeted degradation via direct 26S proteasome recruitment
Nature, Dec 2022
Product used: ProPlus PhyTip® Columns |
Non-viral precision T cell receptor replacement for personalized cell therapy
Nature, Nov 2022
Product used: IMAC PhyTip® Columns
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Distinct Glycosylation Responses to Spinal Cord Injury in Regenerative and Nonregenerative Models
Journal of Proteome Research, May 2022
Product used: Normal Phase PhyTip® Columns
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Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells
New Biotechnology, May 2022
Product used: ProPlus PhyTip® Columns
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Epigenetic comparison of CHO hosts and clones reveals divergent methylation and transcription patterns across lineages
Biotechnology and Bioengineering, Apr 2022
Product used: ProPlus PhyTip® Columns |
Loss of the intracellular enzyme QPCTL limits chemokine function and reshapes myeloid infiltration to augment tumor immunity
Nature, Mar 2022
Product used: Streptavidin PhyTip® Columns |
Structure-guided unlocking of NaX reveals a non-selective tetrodotoxin-sensitive cation channel
Nature, Mar 2022
Product used: C18 PhyTip Columns
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Phosphoproteome Profiling of the Receptor Tyrosine Kinase MuSK Identifies Tyrosine Phosphorylation of Rab GTPases
Molecular & Cellular Proteomics, Feb 2022
Product used: C18 PhyTip Columns
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Rapid discovery of diverse neutralizing SARS-CoV-2 antibodies from large-scale synthetic phage libraries
mAbs, Jan 2022
Product used: Protein A PhyTip® Columns
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| 2021 |
Integrated workflow for urinary prostate specific antigen N-glycosylation analysis using sdAb partitioning and downstream capillary electrophoresis separation
Analytica Chimica Acta, Nov 2021
Product used: IMAC PhyTip® Columns
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Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
Nature, Jul 2021
Product used: ProPlus PhyTip® Columns |
An end-to-end automated platform process for high-throughput engineering of next-generation multi-specific antibody therapeutics
mAbs, Apr 2021
Product used: Protein A PhyTip® Columns, IMAC PhyTip® Columns, Miniprep PhyTip Columns |
Functional GLP-1R antibodies identified from a synthetic GPCR-focused library demonstrate potent blood glucose control
mAbs, Mar 2021
Product used: Protein A PhyTip® Columns |
PD-L1+ neutrophils contribute to injury-induced infection susceptibility
SCIENCE ADVANCES, Mar 2021
Product used: ProPlus PhyTip® Columns, CaptureSelect PhyTip Columns |
Activity of murine surrogate antibodies for durvalumab and tremelimumab lacking effector function and the ability to deplete regulatory T cells in mouse models of cancer
mAbs, Jan 2021
Product used: ProPlus PhyTip® Columns, CaptureSelect PhyTip Columns |
| 2020 |
High-throughput, parallelized and automated protein purification for therapeutic antibody development
Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics, 2020
Product used: Protein A PhyTip® Columns |
Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost-effective method for expression of complex molecules
Biotechnology Journal, Mar 2021
Product used: Protein A PhyTip® Columns |
| 2019 |
Association of an HDL Apolipoproteomic Score With Coronary Atherosclerosis and Cardiovascular Death
Journal of the American College of Cardiology, May 2019
Product used: PhyTip Columns with custom resins |
Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins
High-Throughput Protein Production and Purification, Jul 2019
Product used: ProPlus PhyTip® Columns |
High-Throughput Protein Production and Purification Methods and Protocols
High-Throughput Protein Production and Purification Book, Jul 2019
Product used: ProPlus PhyTip® Columns |
Perfusion cell culture for the production of conjugated recombinant fusion proteins reduces clipping and quality heterogeneity compared to batch-mode processes
Journal of Biotechnology, Aug 2019
Product used: PhyTip Columns with custom resins |
| Salivary N-glycosylation as a biomarker of oral cancer: A pilot study |
| Shedding Light into the Subcutis: A Mass Spectrometry Based Immunocapture Assay Enabling Full Characterization of Therapeutic Antibodies after Injection in Vivo |
| DNA-Encoded Macrocyclic Peptide Library |
| Direct imaging of the recruitment and phosphorylation of S6K1 in the mTORC1 pathway in living cells |
| 2018 |
| Impact of cell culture media additives on IgG glycosylation produced in Chinese hamster ovary cells |
| Quantitative Assessment of Affinity Selection Performance by Using DNA-Encoded Chemical Libraries |
| Development of a Selection Method for Discovering Irreversible (Covalent) Binders from a DNA-Encoded Library |
| Automated high throughput microscale antibody purification workflows for accelerating antibody discovery |
| Pyruvate Kinase Muscle-1 Expression Appears to Drive Lactogenic Behavior in CHO Cell Lines, Triggering Lower Viability and Productivity: A Case Study |
| Critical considerations for immunocapture enrichment LC-MS bioanalysis of protein therapeutics and biomarkers |
| Development of a shake tube-based scale-down model for perfusion cultures |
| Conservation of oncofetal antigens on human embryonic stem cells enables discovery of monoclonal antibodies against cancer |
| Improved Performance in Mammalian Cell Perfusion Cultures by Growth Inhibition |
| Structure-Guided Combinatorial Engineering Facilitates Affinity and Specificity Optimization of Anti-CD81 Antibodies |
| Multi-step high-throughput conjugation platform for the development of antibody-drug conjugates |
| Design and Application of a DNA-Encoded Macrocyclic Peptide Library |
| Controlling the Glycosylation Profile in mAbs Using Time-Dependent Media Supplementation |
| Agonists and Antagonists of Protease-Activated Receptor 2 Discovered within a DNA-Encoded Chemical Library Using Mutational Stabilization of the Target |
| Randomness in DNA Encoded Library Selection Data Can Be Modeled for More Reliable Enrichment Calculation |
| 2017 |
| Use of a protein engineering strategy to overcome limitations in the production of "Difficult to Express" recombinant proteins |
| Auto-induction of Pichia pastoris AOX1 promoter for membrane protein expression |
| Streamlining workflow and automation to accelerate laboratory scale protein production |
| The Tetrameric Plant Lectin BanLec Neutralizes HIV through Bidentate Binding to Specific Viral Glycans |
| Modulation and modeling of monoclonal antibody N-linked glycosylation in mammalian cell perfusion reactors |
| Characterization of H type 1 and type 1 N-acetyllactosamine glycan epitopes on ovarian cancer specifically recognized by the anti-glycan monoclonal antibody mAb-A4 |
| Capillary electrophoresis analysis of N-glycosylation changes of serum paraproteins in multiple myeloma |
| Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis |
| Discovery of a Potent BTK Inhibitor with a Novel Binding Mode by Using Parallel Selections with a DNA-Encoded Chemical Library |
| Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach |
| 2016 |
| Efficient Qualitative and Quantitative Determination of Antigen-induced Immune Responses |
| CP40 from Corynebacterium pseudotuberculosis is an endo-β-N-acetylglucosaminidase |
| Twoplex 12/13 C6 aniline stable isotope and linkage-specific sialic acid labeling 2D-LC-MS workflow for quantitative N-glycomics |
| Discovery of cofactor-specific, bactericidal Mycobacterium tuberculosis InhA inhibitors using DNA-encoded library technology |
| Robust factor selection in early cell culture process development for the production of a biosimilar monoclonal antibody |
| Process performance and product quality in an integrated continuous antibody production process |
| Identifying a robust design space for glycosylation during monoclonal antibody production |
| EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae |
| High-throughput analysis of immunoglobulin G glycosylation |
| Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches |
| Rapid N-glycan release from glycoproteins using immobilized PNGase F microcolumns |
| Implementation of an Automated High-Throughput Plasmid DNA Production Pipeline |
| From haystack to needle: finding value with DNA encoded library technology at GSK† |
| Expression, purification, and characterization of recombinant human and murine milk fat globule-epidermal growth factor-factor 8 |
| Automated screening for small organic ligands using DNA-encoded chemical libraries |
| The Type IV Pilus Assembly ATPase PilB of Myxococcus xanthus Interacts with the Inner Membrane Platform Protein PilC and the Nucleotide-binding Protein PilM |
| Evaluating high-throughput scale-down chromatography platforms for increased process understanding |
| A Combination of Structural and Empirical Analyses Delineates the Key Contacts Mediating Stability and Affinity Increases in an Optimized Biotherapeutic Single-chain Fv (scFv) |
| Lessons learned in building high-throughput process development capabilities |
| 2015 |
| Current advances in the development of high-throughput purification strategies for the generation of therapeutic antibodies |
| DNA Encoded Library Selections and Insights Provided by Computational Simulations |
| High-throughput purification tools for rapid upstream process development are interchangeable for biologics |
| Improved assembly of bispecific antibodies from knob and hole half-antibodies |
| Comparative glycoprofiling of HIV gp120 immunogens by capillary electrophoresis and MALDI mass spectrometry |
| Encoded library technology screening of hepatitis C virus NS4B yields a small-molecule compound series with in vitro replicon activity |
| Poly(N-vinylimidazole/ethylene glycol dimethacrylate) for the purification and isolation of phenolic acids |
| Use of the site-specific retargeting jump-in platform cell line to support biologic drug discovery |
| A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems |
| Sample preparation for N-glycosylation analysis of therapeutic monoclonal antibodies by electrophoresis |
| Generation of potent mouse monoclonal antibodies to self-proteins using T-cell epitope "tags" |
| Augmented Binary Substitution: Single-pass CDR germ-lining and stabilization of therapeutic antibodies |
| Optimisation of a simple method to transiently transfect a CHO cell line in high-throughput and at large scale |
| Structure of a highly acidic β-lactamase from the moderate halophile Chromohalobacter sp. 560 and the discovery of a Cs(+)-selective binding site |
| Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris |
| Identification of anti-tumour biologics using primary tumour models, 3-D phenotypic screening and image-based multi-parametric profiling |
| Arginine methylation and citrullination of splicing factor proline- and glutamine-rich (SFPQ/PSF) regulates its association with mRNA |
| 2014 |
| Use of a protein engineering strategy to overcome limitations in the production of "Difficult to Express" recombinant proteins |
| Auto-induction of Pichia pastoris AOX1 promoter for membrane protein expression |
| Streamlining workflow and automation to accelerate laboratory scale protein production |
| The Tetrameric Plant Lectin BanLec Neutralizes HIV through Bidentate Binding to Specific Viral Glycans |
| Modulation and modeling of monoclonal antibody N-linked glycosylation in mammalian cell perfusion reactors |
| Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve biosimilar product quality |
| Characterization of H type 1 and type 1 N-acetyllactosamine glycan epitopes on ovarian cancer specifically recognized by the anti-glycan monoclonal antibody mAb-A4 |
| Capillary electrophoresis analysis of N-glycosylation changes of serum paraproteins in multiple myeloma |
| Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis |
| Discovery of a Potent BTK Inhibitor with a Novel Binding Mode by Using Parallel Selections with a DNA-Encoded Chemical Library |
| Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach |
| 2013 |
| Strategy for selecting disposable bags for cell culture media applications based on a root-cause investigation |
| The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody |
| Combining phenotypic and proteomic approaches to identify membrane targets in a 'triple negative' breast cancer cell type |
| CDR-restricted engineering of native human scFvs creates highly stable and soluble bifunctional antibodies for subcutaneous delivery |
| Automated sample preparation for CE-SDS |
| Facile fabrication and instant application of miniaturized antibody-decorated affinity columns for higher-order structure and functional characterization of TRIM21 epitope peptides |
| Strategy for selecting disposable bags for cell culture media applications based on a root-cause investigation |
| The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody |
| Combining phenotypic and proteomic approaches to identify membrane targets in a 'triple negative' breast cancer cell type |
| CDR-restricted engineering of native human scFvs creates highly stable and soluble bifunctional antibodies for subcutaneous delivery |
| 2012 |
| High-throughput process development of chromatography steps: advantages and limitations of different formats used |
| Method for recovery and immunoaffinity enrichment of membrane proteins illustrated with metastatic ovarian cancer tissues |
| 2010 |
| Chip-based CE for rapid separation of 8-aminopyrene-1,3,6-trisulfonic acid (APTS) derivatized glycans |
| Mass spectrometric-based stable isotopic 2-aminobenzoic acid glycan mapping for rapid glycan screening of biotherapeutics |
| Widening the bottleneck: increasing success in protein expression and purification |
| 2009 |
| RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography |
| Ion Chromatography, 4th, Completely Revised and Enlarged Edition |
| Fluorescent isotope-coded affinity tag 2: peptide labeling and affinity capture |
| Enrichment of Amadori products derived from the nonenzymatic glycation of proteins using microscale boronate affinity chromatography |
| High-throughput immunoglobulin G N-glycan characterization using rapid resolution reverse-phase chromatography tandem mass spectrometry |
| 2008 |
| Fluorescent isotope-coded affinity tag (FCAT). I: Design and synthesis |
| A simple high-throughput purification method for hit identification in protein screening |
| Selection against undifferentiated human embryonic stem cells by a cytotoxic antibody recognizing podocalyxin-like protein-1 |
| Boronic acid lectin affinity chromatography (BLAC). 2. Affinity micropartitioning-mediated comparative glycosylation profiling |
| Sample preparation for the analysis of complex carbohydrates by multicapillary gel electrophoresis with light-emitting diode induced fluorescence detection |
| A microscale yeast cell disruption technique for integrated process development strategies |
| 2007 |
| Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution |
| Automated sample preparation facilitated by PhyNexus MEA purification system for oligosaccharide mapping of glycoproteins |
| An automated microscale chromatographic purification of virus-like particles as a strategy for process development |
| 2006 |
| High-throughput affinity ranking of antibodies using surface plasmon resonance microarrays |
| Material-enhanced laser desorption/ionization (MELDI)--a new protein profiling tool utilizing specific carrier materials for time of flight mass spectrometric analysis |
| 2005 |
| First meeting on Developments in Protein Interaction Analysis (DiPIA 2005). August 28-31, 2005, PA, USA |
| Striving for purity: advances in protein purification |
| Protein purification: pure but not simple |
| |
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