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While many of the standard amino acids can be purchased pre-loaded onto Wang type resins, there are still cases where coupling the first amino acid onto Wang resin manually is necessary. In my case, a...
For many synthetic chemists the primary purification goal is to isolate as much synthetic product as possible with a minimum of 80% purity. The go-to technique for product isolation is flash purificat...
Purifying your synthetic product efficiently in high yield with minimal impurities is every chemist’s goal. At discovery-scale, flash chromatography is the go-to purification technique as it is relati...
For chemists isolating their synthesized product in maximum yield and purity is a primary goal. Sometimes the crude reaction mixture stays in solution, sometimes it does not. In these cases, is it bet...
For most chemists purifying organic reaction mixtures, normal-phase flash chromatography is the go-to technique. Why not, it is quick, relatively efficient, and can provide relatively high loading cap...
Flash chromatography is a purification technique used by chemists to isolate their targeted compound from by-products and impurities. Because the reaction mixture (or natural product extract) may have...
A good question I get asked frequently to which there is no specific value. When asked this question I answer, “it depends on your sample and how good your separation is”; not a satisfying response, b...
We’ve all done it. Loaded a very small amount of our peptide onto an analytical, maybe semi-prep column, to see where the desired peak elutes, then create a shortened version of that gradient for the ...
Let's be honest. Optimizing a purification protocol for a single peptide is not something that one wants to spend a lot of time thinking about, let alone actually pursuing. There are times though, tha...
Disulfide rich peptides are unique in both their incredibly high cysteine content, but also in the stability imbued by the multiple disulfide bonds. These peptides, stable under extreme conditions tha...