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Aspartimide rearrangements are a particularly nasty side reaction that can occur during fmoc-based solid phase peptide synthesis. Not only is this a mass-neutral side reaction, chromatographically res...
Almost all the peptides I have synthesized were subsequently purified using a reversed-phase C18 column. Sometimes this worked, but sometimes it didn’t work so well. When my C18 purifications failed, ...
Ion pairing agents are used in a variety of strategies to improve overall purification efficiency.
We've all used mass spectrometry to characterize our synthetic peptides. It's often used to confirm that the peptide was in fact synthesized, then again as part of the purification process to make sur...
Since the development of Fmoc-based solid phase peptide synthesis, a wide variety of cleavage cocktails have emerged. Each cleavage cocktail contains a unique combination of scavengers designed to pre...
Whether it’s the bonded stationary phase, particle size, or even particle pore size, scientists today are offered a plethora of choices when it comes to reversed phase HPLC columns. An often acknowled...
We all use mobile phase modifiers, regardless of purification strategy, for purification of our synthetic peptides. With ionizable side chains, something that affects the pH of the mobile phase is a m...
When purifying crude peptides, we always use a mobile phase modifier. The modifier simplifies a potentially complex purification by driving the desired peptide, and any impurities in the sample, into ...
The isoelectric point (pI) is a physical property of every peptide (and any other compound for that matter) that can be the root cause for many of the issues experienced when handling these compounds....
Analytical laboratories using techniques such as LC-MS/MS or GC-MS are continuously searching for ways to increase throughput, reduce turnaround times and lower costs. Sample prep is often an underest...