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Often the question arises asking how can I extract both acids and bases with the same Supported Liquid Extraction (SLE) procedure. Is this possible? And how?

Unbiased peptide screening technologies have evolved, improved and recently gained acceptance by the community as strategies for identifying new lead compounds against old targets with in novel chemic...

Preparative HPLC and flash chromatography are two of the most utilized techniques for purifying molecules post synthesis. To monitor sample purity and elution times, detection techniques such as UV/UV...

Evaporative light-scattering detection (ELSD) is an effective tool for detecting compounds with minimal to no UV absorbance. ELS detectors convert the column effluent into an aerosol by adding heated ...

I like working with natural products, at least from a chromatography perspective. The sheer breadth of compounds extractable from a plant makes separations a challenge but overcoming that challenge wi...

ELSD, or evaporative light scattering detector, is an accessory item available for many commercially available flash chromatography systems. It is typically used to detect compounds that are UV transp...

Cl-trityl resin can be tricky to use not only from an amino acid loading perspective, but one must also consider the highly labile linkage to the solid support1. This lability is advantageous in insta...

Dry loading crude reaction mixtures can dramatically improve purification results. This is a topic I have blogged about several times and is important enough to discuss again.

Are you currently or have you ever had an issue where your chromatographic trace shows a sine-wave pattern while a compound is eluting, Figure 1? If so, there are several causes, and solutions, for th...

The answer to this question is absolutely yes. The choice of organic solvent can impact your reversed phase flash purification results just as organic solvent selection impacts normal phase results.