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Cl-trityl resin can be tricky to use not only from an amino acid loading perspective, but one must also consider the highly labile linkage to the solid support1. This lability is advantageous in insta...
Dry loading crude reaction mixtures can dramatically improve purification results. This is a topic I have blogged about several times and is important enough to discuss again.
Are you currently or have you ever had an issue where your chromatographic trace shows a sine-wave pattern while a compound is eluting, Figure 1? If so, there are several causes, and solutions, for th...
The answer to this question is absolutely yes. The choice of organic solvent can impact your reversed phase flash purification results just as organic solvent selection impacts normal phase results.
If you use automated flash chromatography you may have a system than can have four solvents attached to it. In fact, most flash chromatography systems are designed to have as many as four solvents ava...
Finding the right affinity tag can be a challenging task for many. The process requires not only an understanding of your protein but also the downstream process of your purification. You need to find...
Head-to-tail cyclization of peptides is being used in drug development as yet another way to probe how constraining peptide sequence structure in solution can improve pharmacokinetics and target speci...
We all know the adage “Time is Money”. It is particularly true in pharma where the timeline to get a pharmaceutical product to market can take 10+ years and cost billions of dollars. With this as the ...
The big question…what affects cyclization efficiency? This is what I have been trying to answer over the course of the last few experiments, especially when it comes to head-to-tail macrocycles. I hav...
With the increasing use of reversed phase flash chromatography in medicinal chemistry and organic synthesis labs comes the desire to speed up and make these purifications more time and solvent efficie...