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Have you ever had a purified reaction product, which should be white, retain color after purification? If so, what did you do to remove it?

With the increased use of reversed phase flash chromatography for reaction mixture purification comes a lot of questions since many chemists are not as familiar with this technique as they are with no...

Flash chromatography is a great tool to purify reaction mixtures and natural product extracts. Modern flash chromatography instrumentation incorporates UV detectors to see eluting compounds and trigge...

The dynamics of solid phase peptide synthesis at the resin surface makes it particularly difficult to create head to tail macrocycles. Dimerization, steric hinderance, and C-terminal access all reduce...

Silica dissolution in methanol is a concern many chemists have. Over my career I have heard that complaint probably hundreds of times which invariably leads to the question - how much methanol can I u...

Flash chromatography with real-time UV detection and fractionation is the primary tool of chemists for isolating synthetic compounds. However, many commonly used flash chromatography solvents (ethyl a...

What is bead mill homogenization? Well, first we need to understand that homogenization is the process of making something uniform. So, what is bead mill homogenization? Bead mill homogenization is a ...

If you synthesize organic compounds you likely have encountered situations where traditional silica or even reversed-phase chromatography just does not get the job done. Co-elution, peak tailing, yiel...

Macrocyclization of peptides has arisen as a new strategy to create proteolytically stable, membrane permeable drug candidates while maintaining target specificity. To perform this cyclization, lactam...

For chemists trying to purify polar compound mixtures, optimizing the chromatography can be very challenging. When hexane (or heptane) with ethyl acetate and a silica column fails to effectively purif...