Can I make head-to-tail macrocycles on-resin?
Macrocyclization of peptides has arisen as a new strategy to create proteolytically stable, membrane permeable drug candidates while maintaining target specificity. To perform this cyclization, lactam...
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What are good alternatives to shallow DCM/MeOH gradients?
For chemists trying to purify polar compound mixtures, optimizing the chromatography can be very challenging. When hexane (or heptane) with ethyl acetate and a silica column fails to effectively purif...
How do strongly basic analytes and SPE EVOLUTE®WCX work?
We know that compounds can be acidic, basic and neutral. Looking at pKas and functional groups can help us understand the best approach for SPE selection and workflow. Let’s take a look at the compoun...
How do weakly acidic analytes and EVOLUTE®AX work?
We know that compounds can be acidic, basic and neutral. Looking at pKas and functional groups can help us understand the best approach for SPE selection. Let’s take a look at the compound, methylmalo...
How to effectively use your flash system's diode-array detector
Virtually all automated flash chromatography systems today contain a diode-array UV or UV-vis detector (DAD). This type of UV detector offers multiple benefits over monochromatic UV detectors includin...
How do weakly basic analytes and SPE EVOLUTE®CX work?
We know that compounds can be acidic, basic, and neutral. Looking at pKa and functional groups can help us further in selecting the right SPE sorbent and better understanding how it works with our ana...
What can I use to purify polar reaction mixtures?
Polar reaction mixtures can be a real challenge to purify with flash chromatography. Traditional normal-phase flash requires toxic dichloromethane (DCM) or chloroform with methanol for these types of ...
How do I know what the best QuEChERS cleanup mix is for my samples?
You may have already read the previous blogs about QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction and cleanup, but, if you have quite a few different matrix interferences or analyte...
Automated pull-down enrichment for biomolecule separation in complex samples
In unconventional assays, it may be difficult to find an effective purification method to separate your biomolecule from your complex sample. With factors such as specificity, selectivity, stability, ...
How do neutral analytes and SPE EVOLUTE® ABN work?
We know that compounds can be acidic, basic, and neutral. Looking at pKas and functional groups can help us understand the best approach and steps for SPE selection and workflow. Let’s look at the com...