PhyTip columns with CaptureSelect™ KappaXl affinity media can be used for purification of human antibodies as it binds with high specificity to human kappa light chains. This affinity media was specifically designed to purify all human kappa light chains (subclass I, II, III and IV) containing antibody formats and fragments, including kappa Fab, kappa Dab2’ and all subclasses of human kappa IgG, IgA, IgD, IgE and IgM.
The affinity ligands of CaptureSelect™ medias from ThermoFisher are based on a recombinant antibody fragment of only 14 kDa in size that can be adapted for highly selective binding to certain parts or antibodies and antibody fragments.
PhyNexus, now part of Biotage, developed the PhyTip® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step.
Due to maximization of binding of the sample, less protein is lost during the purification process. The result is very high sample recoveries.
Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.
Effective purification by PhyTip columns enables a full purification protocol of 96 samples in as little as 15 minutes.
Our PhyTip columns are available for all major liquid handling platforms to enable you to use your existing system that you may already have in your lab for automated protein purification. If you don't have a system, we offer automation solutions for protein purification by PhyTip columns.
When choosing a PhyTip® column for your purification process, the optimal resin bed volume of the column depends on the total amount of protein present in your samples. If you are working with very low protein concentrations of 10 μg/mL or lower, we recommend using a resin bed volume one or two sizes bigger than indicated below for an effective purification process.
We offer PhyTip® columns available for all major liquid handling systems. Download here our guide over PhyTip® column compatibility with different liquid handling systems.
If you are not sure which PhyTip® column will that work best in your protocol, our specialists are here to help you.
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
PTH-91-16-31 | CaptureSelect KappaXL PhyTip columns (1 mL volume) containing 160 uL affinity media resin per column, Hamilton | 96 | Add to quote/cart | |
PTB-91-10-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman | 96 | 1139 USD | Add to quote/cart |
PTX-91-10-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix | 96 | 1139 USD | Add to quote/cart |
PTH-91-10-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton | 96 | 1139 USD | Add to quote/cart |
PTR-91-10-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA 2 | 96 | 1139 USD | Add to quote/cart |
PTT-91-10-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan | 96 | 1139 USD | Add to quote/cart |
PTB-91-20-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman | 96 | 1603 USD | Add to quote/cart |
PTX-91-20-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 1603 USD | Add to quote/cart |
PTH-91-20-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton | 96 | 1603 USD | Add to quote/cart |
PTR-91-20-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA 2 | 96 | 1603 USD | Add to quote/cart |
PTT-91-20-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan | 96 | 1603 USD | Add to quote/cart |
PTH-91-80-31 | CaptureSelect™ KappaXL PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton | 96 | 5711 USD | Add to quote/cart |
PTV-92-20-31 | CaptureSelect™ KappaXL PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo | 96 | 1603 USD | Add to quote/cart |
PTB-92-20-31 | CaptureSelect™ KappaXL PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman | 96 | 1603 USD | Add to quote/cart |
PTR-92-20-31 | CaptureSelect™ KappaXL PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2 | 96 | 1603 USD | Add to quote/cart |
PTT-92-20-31 | CaptureSelect™ KappaXL PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 1603 USD | Add to quote/cart |
PTB-92-05-31 | CaptureSelect™ KappaXL PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman | 96 | 768 USD | Add to quote/cart |
PTR-92-05-31 | CaptureSelect™ KappaXL PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2 | 96 | 768 USD | Add to quote/cart |
PTT-92-05-31 | CaptureSelect™ KappaXL PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan | 96 | 768 USD | Add to quote/cart |
PTX-93-20-31 | CaptureSelect™ KappaXL PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 1603 USD | Add to quote/cart |
PTH-93-20-31 | CaptureSelect™ KappaXL PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton | 96 | 1603 USD | Add to quote/cart |
PTX-93-05-31 | CaptureSelect™ KappaXL PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix | 96 | 768 USD | Add to quote/cart |
PTH-93-05-31 | CaptureSelect™ KappaXL PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton | 96 | 768 USD | Add to quote/cart |
PTT-95-20-31 | CaptureSelect™ KappaXL PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 1603 USD | Add to quote/cart |
PTT-95-40-31 | POROS CaptureSelect™ KappaXL PhyTip® columns (500 μL volume) containing 40 µL affinity media per column, Tecan | 96 | 3199 USD | Add to quote/cart |
Biotage is the Global Go-To Separations Company, supporting customers from drug discovery and development through to diagnostics and analytical testing with intelligent and sustainable workflow solutions. Our expertise and top-tier separation solutions play a key role in streamlining our customers’ workflows and improving their outcomes. Headquartered in Sweden, Biotage operates globally with 700 employees, serving over 80 countries. Our company is listed on NASDAQ Stockholm (BIOT).
Copyright © 2024 Biotage