Showing posts written by Biotage
A guide to protein purification and workflow automation All research and development stages that involve protein assays require the essential step of protein purification. Regardless if you are workin...
This application note describes a method of extraction for metanephrine and normetanephrine from pooled plasma using EVOLUTE® EXPRESS WCX 96-well SPE plates. Figure 1. Structures metanephrine and norm...
Scope This application note will outline the recommended methods for the extraction of chlorinated biphenyl congeners as outlined in EPA Method 1668A using Biotage automated or manual SPE solutions, A...
Introduction The treatment and removal of oil and grease from wastewater is imperative because it can negatively affect the biological and aquatic life that encounter it. Not only that, but since the ...
Summary and background Purifying reaction mixtures often requires large volumes of solvent and multiple fraction collection vessels, especially when working at larger scales. Greener, more sustainable...
The use of ISOLUTE® HCX mixed-mode sorbents is widely accepted for providing high purity extracts of basic drugs from biological fluids. The dual retention mechanism, provided by non-polar and strong ...
Most drugs and metabolites are conjugated prior to excretion in the urine. Methods to detect drug analytes in urine usually include enzyme hydrolysis to convert glucuronide metabolites back to their f...
Drug screening using oral fluid has gained popularity over recent years due to its simple, non-invasive collection means. Screening drugs of abuse can be complicated due to the wide variation of funct...
Typical bioanalysis workflows for small molecules use organic solvents to precipitate proteins and extract analyte(s). Sometimes, extra clean-up, including liquid-liquid extraction (LLE) or solid phas...
Peptide nucleic acids (PNAs) continue to gain traction in diagnostic and potentially therapeutic applications where oligonucleotides are the current modality of interest. Despite containing an unnatur...