Showing posts written by Biotage
To synthesize cyclic peptides, automated peptide synthesizers using solid phase supports have been the method of choice utilizing cyclization techniques such as lactam and disulfide bridges to impart ...
Synthetic chemistry is hard enough; purifying the reaction product can be even more challenging. If your reaction mixture shows you made your target but also many byproducts, what do you do? You can r...
Routine testing for drugs in urine is commonly performed by clinical, forensic, and pain management laboratories. The sample cleanliness required for analysis by LC-MS/MS can result in lengthy, labor-...
Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation. A fast, reliable and robust sample preparation method that could be...
Oral fluid represents a complex, heterogeneous biological fluid primarily produced by the parotid, submandibular, and sublingual salivary glands. Together, these glands make the majority of saliva, wh...
Drug screening using oral fluid has gained popularity over recent years due to its simple, non-invasive collection means. Screening drugs of abuse can be complicated due to the wide variation of funct...
Historically, silica-based solid phase extraction (SPE) cartridges have been used for drugs of abuse testing, the exact choice being dependent on drug functionality. GC/MS sensitivity requirements tra...
Achieving desired sensitivity of plasma catecholamines by LCMS/MS can be problematic. Low LOQs can require a highly sensitive MS instrument. Evaporation of the extracted sample can result in losses du...
Methylisothiazolinone (MI) is an antimicrobial preservative that is used in a variety of personal care products, such as sunscreens, lotions, cosmetics. MI is a cytotoxin and as a result there is conc...
Protein precipitation is a routinely used, high throughput sample preparation technique for removal of protein from biological fluid (plasma, serum or blood) samples prior to analysis by LC-MS/MS. His...