Showing posts written by Biotage
In medicinal chemistry each intermediate and final product needs purification. Historically, the primary tool for reaction purification has been normal-phase flash chromatography sometimes followed by...
Evaporative crosstalk is well-to-well cross contamination during extract evaporation after SLE or SPE extraction in a 96 well plate. It is often observed with volatile analytes like methamphetamine an...
The opioid crisis in the United States has necessitated monitoring of these drugs in biological fluids by many state health laboratories. Urine is a common matrix for analysis, and labs require a simp...
Traditionally, peptide purification has relied on preparative high performance liquid chromatography (prep-HPLC), known for its excellent resolution and impurity removal. However, this method is often...
Estrogen, androgen, and glucocorticoid metabolism can be used to assess overall hormonal balance during hormone therapy. Urine is the recommended testing matrix for quantification of primary estrogen ...
Part of the B complex group of water soluble vitamins, vitamin B7 is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioana...
Urine drug testing to support pain management is a mainstay of the clinical toxicology laboratory. Reduced reimbursement has continued to put increasing pressure on laboratories. Many toxicology labs ...
Solid phase peptide synthesis (SPPS) is used in the production of more than 80% of commercially manufactured peptides, and to an even greater extent at smaller scales. With the passing of the REACH Ac...
Dilute and shoot (D/S) is the most common form of sample preparation for the analysis of drugs of abuse in urine. High analyte cut-offs combined with sensitive mass spectrometers allow substantial sam...
Flash chromatography is the primary tool used by medicinal chemists for reaction mixture purification. Over the past 10 or so years, the use of reversed-phase flash chromatography for reaction mixture...