|Chemical description||Primary/secondary amine modified polystyrene-divinylbenzene incorporating non-ionizable hydroxyl groups|
|Sorbent type||Mixed-mode non-polar/weak anion exchange|
|Exchange capacity||0.3 mmol/g|
|Average particle size||30 µm, 50 µm|
|Pore diameter||40 Å|
This application note describes a Biotage polymer-based mixed-mode weak anion exchange SPE protocol for the extraction of a range of polyfluorinated (PFA) compounds in water prior to LC-MS/MS detection.
High (>80%) reproducible analyte recoveries, with excellent linearity to LOQs of 0.1 ng/L or below are achieved.
Tags: Application Notes, Column, English, Environmental, LC-MS/MS, Perfluorobutanesulfonic acid (PFBuS), Perfluorodecanoic acid (PFDA), Perfluoroheptanoic acid (PFHpA), Perfluorohexanesulfonic acid (PFHxS), Perfluorohexanoic acid (PFHxA), Perfluorononanoic acid (PFNA), Perfluorooctanesulfonic acid (PFOS), Perfluorooctanoic acid (PFOA), Persistant organic pollutants (POP), Polyfluorinated acids, Water
This method describes the use of EVOLUTE WAX plates and columns to extract a suite of organophosphate pesticide metabolites including dimethylphosphate (DMP), dimethylthiophosphate (DMTP), diethylphosphate (DEP), diethylthiophosphate (DETP), dimethyldithiophosphate (DMDTP) and diethyldithiophosphate (DEDTP) from human urine. The method demonstrates extraction of these small polar metabolites at urine concentrations ranging from 40 ng/mL to 400 ng/mL.
resin, polymer, EVOLUTE,
Tags: Application Notes, Clinical, Column, Diethyldithiophosphate (DEDTP), Diethylphosphate (DEP), Diethylthiophosphate (DETP), Dimethyldithiophosphate (DMDTP), Dimethylphosphate (DMP), Dimethylthiophosphate (DMTP), LC-MS/MS, Organophosphate Pesticide, Urine
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches.
EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Tags: 96-well plate, Alcohol metabolite, Column, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Posters, Urine
This technical note provides guidelines for the extraction of strongly acidic compounds from aqueous samples using EVOLUTE WAX. The procedure is described on pages 1, with processing and optimization guidelines on page 2. An example application showing the extraction of pesticide metabolites from urine illustrates the use of EVOLUTE WAX (dimethylthiophosphate (DMTP), diethylphosphate (DEP), hexanesulfonic acid (HSA), benzenesulfonic acid (BSA)) for clean-up of biological fluid samples.
POLYMER, ACIDIC, WEAK ANION EXCHANGER, EVOLUTE, SPE, SAMPLE PREP, SAMPLE PREPARATION, OASIS
Tags: 96-well plate, Acidic drugs, Aqueous, Biological fluids, Column, Diethylphosphate (DEP), Dimethylthiophosphate (DMTP), Drugs of Abuse, LC-MS/MS, Organophosphate Pesticide, Plasma, Product Notes, Serum, Urine
EVOLUTE® WAX mixed mode polymer-based SPE sorbent
extracts a range of acidic analytes from biological fluids and
other aqueous matrices using a generic procedure which
minimizes method development time. EVOLUTE WAX removes
matrix components such as proteins, salts, non-ionisable
interferences and phospholipids, delivering cleaner extracts
with reproducible recoveries for accurate quantitation.
Tags: Biological fluids, Product Notes