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SPE Columns and Plates

Biotage sample preparation column size table ISOLUTE HAX 200 mg/3 mL ISOLUTE HAX 200 mg/3 mL

ISOLUTE® HAX is a mixed-mode sorbent providing clean extracts for acidic compounds from complex biological fluid samples using a dual retention mechanism.


Part No. Description Product type Pack Size Price
903-0010-A ISOLUTE HAX 100 mg/1 mL Consumables 100 Log in for price
903-0020-B ISOLUTE HAX 200 mg/3 mL Consumables 50 Log in for price
903-0020-C ISOLUTE HAX 200 mg/6 mL Consumables 30 Log in for price
903-0020-H ISOLUTE HAX 200 mg/10 mL (3 mL XL) Consumables 50 Log in for price
903-0025-P01 ISOLUTE-96 HAX 25 mg plate Consumables 1 Log in for price

ISOLUTE® HAX combines non-polar (C8) and strong anion exchange retention  (-NR3+mechanisms to isolate acidic analytes from complex aqueous matrices such as urine and plasma.

Analytes are initially retained by a non-polar retention mechanism, with inorganic salts, polar interferences such as phospholipids and large molecular weight peptides and proteins passing directly through the sorbent bed.  The pH is then adjusted to allow an ion exchange retention mechanism to retain the analyte(s).  Non-polar interferences are removed using an organic solvent or organic solvent/water wash.  Analytes are then eluted in an extra clean fraction.

ISOLUTE HAX is available in a wide range of SPE columns and 96-well plate formats.

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