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ISOLUTE® HCX-5

SPE Columns and Plates

ISOLUTE HCX5
Biotage sample preparation column size table

ISOLUTE® HCX-5 is a mixed-mode sorbent providing clean extracts for basic compounds from complex biological fluid samples using a dual retention mechanism.

CONSUMABLES

Part No. Description Product type Pack Size Price
906-0002-A ISOLUTE HCX-5 25 mg/1 mL Consumables 100 Log in for price
906-0010-A ISOLUTE HCX-5 100 mg/1 mL Consumables 100 Log in for price
906-0010-G ISOLUTE HCX-5 100 mg/10 mL (1 mL XL) Consumables 50 Log in for price
906-0013-H ISOLUTE HCX-5 130 mg/10 mL (3 mL XL) Consumables 50 Log in for price

ISOLUTE® HCX-5 combines non-polar (C4) and strong cation exchange retention  (SO3-) mechanisms to isolate basic analytes from complex aqueous matrices such as urine and plasma.

Analytes are initially retained by a non-polar retention mechanism, with inorganic salts, polar interferences such as phospholipids and large molecular weight peptides and proteins passing directly through the sorbent bed. The pH is then adjusted to allow an ion exchange retention mechanism to retain the analyte(s). Non-polar interferences are removed using an organic solvent or organic solvent/water wash. Analytes are then eluted in an extra clean fraction.

ISOLUTE HCX-5 provides the cleanest extracts of all the ISOLUTE mixed-mode sorbents. It is the ideal choice where the analyte to be extracted has sufficient non-polar charater to be well retained by the non-polar (C4) component of the mixed-mode sorbent.

ISOLUTE HCX-5 is available in a wide range of SPE columns and 96-well plate formats.