|Chemical description||Ethylbenzenesulfonic acid (non-endcapped) functionalized silica. Manufactured using a trifunctional silane.|
|Sorbent type||Strong cation exchange|
|Average particle size||50 µm|
|Pore diameter||60 Å|
|Exchange capacity||0.6 meq/g|
This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.
Tags: 96-well plate, Application Notes, B Vitamins, Clinical, English, LC-MS/MS, Niacin (nicotinic acid), Niacinamide, Nicotinuric acid, Serum, Vitamin B3, Vitamins
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
This poster describes a strong cation exchange SPE protocol for extraction of Vitamin B3 (Niacin) and related metabolites from serum.
The ISOLUTE SCX-3 96 well plate format demonstrated as a viable option for serum measurements over a relevant concentration range in clinical diagnostics.
It is anticipated that strategies presented in this poster would be of broad-based interest to clinical laboratories focused on combining parent/metabolite assays into a single analysis.
Tags: 96-well plate, B Vitamins, Clinical, English, LC-MS/MS, Niacin (nicotinic acid), Niacinamide, Nicotinuric acid, Posters, Serum, Vitamin B3, Vitamins
ISOLUTE® Si-TsOH (SCX-3) is the bound equivalent of p-toluene sulfonic acid with a
pKa <1. Similar to ISOLUTE propylsulfonic acid (SCX-2)1 , it can be used to (a) scavenge
amines and other bases such as anilines and borohydrides (b) as an acid catalyst in reactions
or (c) as a replacement for aqueous or organic acids in quenching reactions.
Tags: Product Notes
This technical note includes specifics on the use of ISOLUTE SCX and SCX-3 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchangers (pages 4-5).
Tags: English, Product Notes
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What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|533-0002-A||ISOLUTE SCX-3 25 mg/1 mL||Consumables||100||Log in for price|
|533-0005-A||ISOLUTE SCX-3 50 mg/1 mL||Consumables||100||Log in for price|
|533-0010-A||ISOLUTE SCX-3 100 mg/1 mL||Consumables||100||Log in for price|
|533-0010-B||ISOLUTE SCX-3 100 mg/3 mL||Consumables||50||Log in for price|
|533-0020-B||ISOLUTE SCX-3 200 mg/3 mL||Consumables||50||Log in for price|
|533-0025-P01||ISOLUTE-96 SCX-3 25 mg plate||Consumables||1||Log in for price|
|533-0050-B||ISOLUTE SCX-3 500 mg/3 mL||Consumables||50||Log in for price|
|533-0050-C||ISOLUTE SCX-3 500 mg/6 mL||Consumables||30||Log in for price|
|533-0050-P01||ISOLUTE-96 SCX-3 50 mg plate||Consumables||1||Log in for price|
|533-0100-B||ISOLUTE SCX-3 1 g/3 mL||Consumables||50||Log in for price|
|533-0100-C||ISOLUTE SCX-3 1 g/6 mL||Consumables||30||Log in for price|
|533-0100-P01||ISOLUTE-96 SCX-3 100 mg plate||Consumables||1||Log in for price|
|533-0200-CG||ISOLUTE SCX-3 2 g/6 mL (Tabless)||Consumables||30||Log in for price|
|533-0200-D||ISOLUTE SCX-3 2 g/15 mL||Consumables||20||Log in for price|
|533-0500-E||ISOLUTE SCX-3 5 g/25 mL||Consumables||20||Log in for price|
|533-1000-F||ISOLUTE SCX-3 10 g/70 mL||Consumables||16||Log in for price|