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Polar reaction mixtures can be a real challenge to purify with flash chromatography. Traditional normal-phase flash requires toxic dichloromethane (DCM) or chloroform with methanol for these types of ...
You may have already read the previous blogs about QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction and cleanup, but, if you have quite a few different matrix interferences or analyte...
In unconventional assays, it may be difficult to find an effective purification method to separate your biomolecule from your complex sample. With factors such as specificity, selectivity, stability, ...
We know that compounds can be acidic, basic, and neutral. Looking at pKas and functional groups can help us understand the best approach and steps for SPE selection and workflow. Let’s look at the com...
Silica flash columns are the most frequently used to purify reaction mixtures. Prepacked columns are available from a vast number of vendors as is the silica itself for those wishing to pack their own...
In synthetic organic chemistry, post-reaction work-up using solvent extraction is almost routine. Solvent-solvent extractions using immiscible solvents can remove impurities from the product prior to ...
Flash chromatography is a purification technique that, by its name, is designed to be fast. Faster than open column chromatography and faster than prep HPLC. Flash chromatography is faster due to the ...
Understanding the properties of different SPE sorbents can be confusing, refer to selecting sorbent chemistries for a refresher. But what happens at each step of these different SPE sorbents specifica...
An interesting question for synthesis chemists as the need for reversed-phase flash chromatography for both intermediates and final compounds increases. Historically, normal-phase flash chromatography...
When choosing the stationary phase for SPE understanding the functionality of your target molecule is of critical importance. In order to select the correct SPE chemistry we need to understand how fun...