ISOLUTE® PAH

PAH Extraction

Question:

What concentration of PAH's do I get after strain by means of ISOLUTE 1,5 g PAH filter if i have passed through it 500 mL sample?

Answer:

This is an open ended question as it depends on the initial concentration of the PAH compounds in the water sample and the final volume of your finished extract both of which are unknown. Let me outline with a couple of examples.

Water sample contains various PAHs at 5 ng/L per component

We extract 500 mL through the ISOLUTE PAH column

We elute the analytes from the column using a suitable solvent and then concentrate the resulting eluent

If we concentrated down the eluent to 1 mL final volume we have achieved a x 500 concentration factor and analytes are now present in the final solution at 2.5 ng/mL.

Alternatively

If we concentrated down the eluent to 0.1 mL final volume we have achieved a x 5000 concentration factor an analytes are now present in the final solution at 25 ng/mL.

SPE column capacity

Question:

What is the maximum capacity of an SPE column?

Answer:

The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.

For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.

Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.