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Accessories & Spare partsWhether its difficult matrices like urine, whole blood, oral fluid or hair; broad (and growing) analyte panels, or increasing throughput requirements, our focus is on providing you with a toolbox of smart sample prep solutions.
Learn more and find lots of useful resources for forensic and toxicology applications in the sections below.
This white paper explores different sample preparation techniques and methodologies for over 100 drugs in urine.
Extraction of 49 drugs from urine samples, with elution volume of just 30 µL. Microelution SPE can improve productivity without compromising analyte sensitivity or extract cleanliness.
Urine hydrolysis is performed to eliminate glucuronide drug metabolites before analysis, and it is key part of the sample preparation process. With today's advanced enzymes, hydrolysis can be much faster, and even be performed at room temperature. This poster illustrates how these enzymes can be used in a fully automated hydrolysis and clean up process.
The simple sample preparation procedure, based on a mixedmode/strong cation exchange extraction mechanism, delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 10%.
This application note details an optimized extraction protocol for the use of ISOLUTE SLE+ supported liquid 1 mL sample volume columns to extract a range of benzodiazepines from human urine. This simplified and efficient extraction method has analyte recoveries from 90% with LOQs as low as 40 ng/mL.
The simple sample preparation procedure delivers clean extracts and analyte recoveries of greater than 74% with RSDs lower than 10%. Using the Biotage® Extrahera™, 24
hydrolyzed samples were extracted in under 31 minutes and 24 non-hydrolyzed samples were extracted in under 36 minutes.
The simple sample preparation procedure
delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for carboxy-THC and its deuterated internal standard. Using Biotage® Extrahera™, 24 samples are extracted in approximately 35 minutes.
Among the most widely abused drugs in the world, they are structurally similar and have similar effects to marijuana but are frequently referred to as “legal highs”. How do we analyze for these compounds? How can they be easily extracted from whole blood?
Synthetic cannabinoids are sold under a wide range of names such as K2, Spice, Legal Highs. They are synthesized to circumvent existing laws, and new substances are frequently identified. This method for extraction of and clean up of whole blood samples allows for identification of 12 novel synthetic cannabinoids by LC-MS/MS.
This simple supported liquid extraction method for phosphatidylethanol (PEth) in whole blood does not require complex sample pre-treatment steps, and allows for accurate quantification of 3 common PEth species. The method is fully automatable.
This application note describes the extraction of barbiturate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, benzodiazepines, cocaine and opiates.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL.
This application note describes the extraction of opiate compounds from whole blood, prior to GC/MS analysis.
This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL
This poster demonstrates a simple supported liquid extraction procedure for the extraction of propofol from whole blood suitable for GC-MS analysis. In addition, phospholipid profiles in the extracts were investigated using LC-MS/MS, and showed no breakthrough of these blood components, suggesting the procedure is appropriate for use with LC-MS/MS based analytical procedures.
The simple sample preparation procedure, based on a mixed mode/strong anion exchange extraction mechanism, delivers clean extracts and analyte recoveries greater than 70% with
RSDs lower than 10%. Linearity of greater than 0.99 is achieved from 1–100 ng/mL.
Collection devices contain components such as buffer salts and surfactants which along with typical endogenous oral fluid constituents present particular challenges in sample preparation for LC-MS/MS. This white paper explores strategies to maximize analyte recovery and minimize matrix effects for 85 common drugs of abuse.
This application note describes the extraction of a range of SPICE drugs and metabolites from neat oral fluid and oral fluid from a commercial collection kit using ISOLUTE® SLE+ in both 96-well plate and column formats.
This poster demonstrates protocols for the determination of a range of drugs of abuse following collection with the NeoSal™ oral fluid device and GC/MS analysis. The drug suites includes amphetamines and synthetic cathinones, barbiturates, benzodiazepines, cocaine, opiates, cannabinoids and synthetic cannabinoids.
The methodology delivers clean extracts with analyte recoveries >75% (plate format) or >60% (column format) with %RSD <10% for most analytes and LLOQ from 200 fg/mg of hair.
In poisoning cases, post-mortem sampling of liver may be necessary to determine exposure to toxic substances. This application note demonstrates how to extract and clean up liver samples.
This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL.
This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. Using automation 24 samples are extracted in approximately 35 minutes.
This poster compares different extraction techniques (mixed-mode SPE, Dual mode extraction and supported liquid extraction) for extraction of fentanyl and fentanyl analogues from both urine and whole blood.
This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates.
There are several different extraction methods that can be used to extract synthetic benzodiazepines from biological matrices such as urine and blood. This application note evaluates three common extraction techniques, detailing the methodology used, and comparing results in terms of analyte recovery and matrix effects.
Synthetic cannabinoids are sold under a wide range of names such as K2, Spice and Legal Highs. They are one of the commonest NPS classes. This method for extraction of and clean up of whole blood samples allows for identification of 12 novel synthetic cannabinoids by LC-MS/MS.
This application note describes the extraction of a suite of NBOMe designer drugs from oral fluid matrix with analysis by laser diode thermal desorption (LDTD)-MS/MS.
Synthetic cannabinoids are sold under a wide range of names such as K2, Spice and Legal Highs. They are one of the commonest NPS classes. This method for extraction of and clean up of whole blood samples allows for identification of 12 novel synthetic cannabinoids by LC-MS/MS.
Supported Liquid Extraction plates and columns designed to provide high analyte recoveries, and clean, protein and phospholipid free extracts. Methods are easy to develop, and automation is simple.
Extraction methods and products that can easily handle whole blood samples without complex sample pre-treatment.
Excellent sample clean up while enhancing manual or automated sample preparation workflow and delivering reproducible, high sensitivity analytical results. For elution volumes as low as 20 µL.
Bead mill homogenization for challenging matrices prior to any sample preparation and extraction.
Designed for high throughput laboratories, to provide efficient solvent evaporation in microplate formats.
Biotage is a global supplier and solutions partner to a wide range of customers within drug discovery and development and analytical testing. With strong expertise in separation and purification technologies, and a focus on leveraging intelligent workflow solutions, we aim to efficiently support the advancement and protection of human health.
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