Utilizing 100 mL Sample Volumes for EPA Method 608.3 Automated Extractions on the Biotage® Horizon 5000

The purpose of this application note is to provide a solution for extracting 100 mL of 608.3 wastewater samples utilizing the Biotage® Horizon 5000 to automatically load the sample and rinse the original sample container for solid phase extraction (SPE), DryDisk® Solvent Drying System for drying the residual water from the extracts, and the TurboVap® for concentrating the dried extracts. Samples are extracted in methylene chloride, then solvent exchanged into hexane prior to analysis by Gas Chromatography/Electron Capture Detection (GC/ECD).