The histidine tag is the most commonly used tag for isolation of recombinant proteins. This tag typically consist of six or more histidine amino acids. Immobilized metal affinity chromatography (IMAC) separation resins are used for purification of his-tagged proteins. Elution can be achieved by mild elution with buffer containing imidazole (100 – 250 mM).
Our IMAC PhyTip columns are packed with high capacity Nickel-IMAC affinity resin and are used to purify biologically functional, recombinant His-tagged recombinant proteins and consistently generate purified samples of high purity and concentration by dual flow chromatography.
PhyNexus now part of Biotage developed the PhyTip column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step.