The histidine tag is the most commonly used tag for isolation of recombinant proteins. This tag typically consist of six or more histidine amino acids. Immobilized metal affinity chromatography (IMAC) separation resins are used for purification of his-tagged proteins. Elution can be achieved by mild elution with buffer containing imidazole (100 – 250 mM).
Our IMAC PhyTip columns are packed with high capacity Nickel-IMAC affinity resin and are used to purify biologically functional, recombinant His-tagged recombinant proteins and consistently generate purified samples of high purity and concentration by dual flow chromatography.
PhyNexus now part of Biotage developed the PhyTip column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step.
Our IMAC PhyTip columns are packed with a high capacity IMAC resin, which routinely generates purified samples of his-tagged protein with 60 - 80% sample recovery.
Our IMAC PhyTip columns routinely produce his-tagged protein samples of >90% purity as measured by the absence of background proteins present in the purified sample.
Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.
PhyTip columns process samples by dual flow chromatography which is a gentle purification process for high levels of biologically active protein.
When choosing a PhyTip® column for your purification process, the optimal resin bed volume of the column depends on the total amount of protein present in your samples. If you are working with very low protein concentrations of 10 μg/mL or lower, we recommend using a resin bed volume one or two sizes bigger than indicated below for an effective purification process.
We offer PhyTip® columns available for all major liquid handling systems.
If you are not sure which PhyTip® column will that work best in your protocol, our specialists are here to help you.
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
PTR-92-20-NS | Box of 96 PhyScreen IMAC Resin Screening Toolkit containing 20 µL of 6 IMAC resins, MEA | 96 | 1922 USD | Add to quote/cart |
PTR-92-05-41 | Box of 96 PhyTip® columns (0.2 mL volume) containing 5 µL Ni-NTA His Bind Superflow resin per column, MEA | 96 | 617 USD | Add to quote/cart |
PTT-92-05-41 | Box of 96 PhyTip® columns (0.2 mL volume) containing 5 µL Ni-NTA His Bind Superflow resin per column. Tecan Te-MO | 96 | 672 USD | Add to quote/cart |
PTT-92-05-50 | Box of 96 PhyTip® columns (200 µL volume) containing 5 µL of Hi Affiniity Ni-IDA resin per column, Tecan Te-MO. | 96 | 672 USD | Add to quote/cart |
PTV-92-20-NS | Custom Box of 96 PhyScreen Ni-IMAC Resin Screening Toolkit containing 20 µL of 12 IMAC resins, Agilent | 96 | 1995 USD | Add to quote/cart |
PTB-92-05-BW | CUSTOM Box of 96 PhyTip® columns (0.2 mL volume) containing 5 µL Ni-NTA (Qiagen) resin per column, Beckman. | 96 | 752 USD | Add to quote/cart |
PTB-92-05-BW-BWB | CUSTOM Box of 96 PhyTip® columns (0.2 mL volume) containing 5 µL Ni-NTA (Qiagen) resin per column, Beckman. | 96 | 752 USD | Add to quote/cart |
PTH-93-20-BW | CUSTOM Box of 96 PhyTip® columns (0.3 mL volume) containing 20 µL Ni-NTA (Qiagen) resin per column, Hamilton | 96 | 877 USD | Add to quote/cart |
PTH-93-05-BW | CUSTOM Box of 96 PhyTip® columns (0.3 mL volume) containing 5 µL Ni-NTA (Qiagen) resin per column, Hamilton | 96 | 752 USD | Add to quote/cart |
PTH-91-10-BW | CUSTOM Box of 96 PhyTip® columns (1 mL volume) containing 10 µL Ni-NTA (Qiagen) resin per column, Hamilton | 96 | 752 USD | Add to quote/cart |
BUF-20-00-03 | IMAC PhyTips® Buffer kit (20 Box Kit) | 1 | 278 USD | Add to quote/cart |
BUF-20-00-03 | IMAC PhyTips® Buffer kit (20 Box Kit) | 1 | 278 USD | Add to quote/cart |
BUF-05-00-03 | IMAC PhyTips® Buffer kit (5 Box Kit) | 1 | 107 USD | Add to quote/cart |
BUF-05-00-03 | IMAC PhyTips® Buffer kit (5 Box Kit) | 1 | 107 USD | Add to quote/cart |
BUF-01-00-03 | IMAC PhyTips® Buffer kit (Single Box Kit) | 1 | 55 USD | Add to quote/cart |
BUF-01-00-03 | IMAC PhyTips® Buffer kit (Single Box Kit) | 1 | 55 USD | Add to quote/cart |
PTB-91-10-03 | IMAC PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman | 96 | 719 USD | Add to quote/cart |
PTX-91-10-03 | IMAC PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix | 96 | 719 USD | Add to quote/cart |
PTH-91-10-03 | IMAC PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton | 96 | 719 USD | Add to quote/cart |
PTR-91-10-03 | IMAC PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA2/ME | 96 | 719 USD | Add to quote/cart |
PTR-91-10-03 | IMAC PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA2/ME | 96 | 719 USD | Add to quote/cart |
PTT-91-10-03 | IMAC PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan | 96 | 719 USD | Add to quote/cart |
PTB-91-16-03 | IMAC PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman | 96 | 2463 USD | Add to quote/cart |
PTX-91-16-03 | IMAC PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix | 96 | 2463 USD | Add to quote/cart |
PTX-91-16-03 | IMAC PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix | 96 | 2463 USD | Add to quote/cart |
PTH-91-16-03 | IMAC PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton | 96 | 2463 USD | Add to quote/cart |
PTR-91-16-03 | IMAC PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA2/ME | 96 | 2463 USD | Add to quote/cart |
PTR-91-16-03 | IMAC PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA2/ME | 96 | 2463 USD | Add to quote/cart |
PTT-91-16-03 | IMAC PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan | 96 | 2463 USD | Add to quote/cart |
PTB-91-20-03 | IMAC PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman | 96 | 827 USD | Add to quote/cart |
PTX-91-20-03 | IMAC PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 827 USD | Add to quote/cart |
PTX-91-20-03 | IMAC PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 827 USD | Add to quote/cart |
PTH-91-20-03 | IMAC PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton | 96 | 827 USD | Add to quote/cart |
PTR-91-20-03 | IMAC PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA2/ME | 96 | 827 USD | Add to quote/cart |
PTR-91-20-03 | IMAC PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA2/ME | 96 | 827 USD | Add to quote/cart |
PTT-91-20-03 | IMAC PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan | 96 | 827 USD | Add to quote/cart |
PTR-91-32-03 | IMAC PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA2/ME | 96 | 2840 USD | Add to quote/cart |
PTX-91-40-03 | IMAC PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix | 96 | 1168 USD | Add to quote/cart |
PTX-91-40-03 | IMAC PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix | 96 | 1168 USD | Add to quote/cart |
PTH-91-40-03 | IMAC PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton | 96 | 1168 USD | Add to quote/cart |
PTR-91-40-03 | IMAC PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA2/ME | 96 | 1168 USD | Add to quote/cart |
PTT-91-40-03 | IMAC PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan | 96 | 1168 USD | Add to quote/cart |
PTB-91-80-03 | IMAC PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman | 96 | 1851 USD | Add to quote/cart |
PTX-91-80-03 | IMAC PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix | 96 | 1851 USD | Add to quote/cart |
PTX-91-80-03 | IMAC PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix | 96 | 1851 USD | Add to quote/cart |
PTH-91-80-03 | IMAC PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton | 96 | 1851 USD | Add to quote/cart |
PTR-91-80-03 | IMAC PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA2/ME | 96 | 1851 USD | Add to quote/cart |
PTT-91-80-03 | IMAC PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan | 96 | 1851 USD | Add to quote/cart |
PTV-92-20-03 | IMAC PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo | 96 | 827 USD | Add to quote/cart |
PTB-92-20-03 | IMAC PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman | 96 | 827 USD | Add to quote/cart |
PTR-92-20-03 | IMAC PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA2/ME | 96 | 827 USD | Add to quote/cart |
PTT-92-20-03 | IMAC PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 827 USD | Add to quote/cart |
PTB-92-05-03 | IMAC PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman | 96 | 587 USD | Add to quote/cart |
PTR-92-05-03 | IMAC PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA2/ME | 96 | 587 USD | Add to quote/cart |
PTT-92-05-03 | IMAC PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan | 96 | 587 USD | Add to quote/cart |
PTX-93-20-03 | IMAC PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 827 USD | Add to quote/cart |
PTX-93-20-03 | IMAC PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 827 USD | Add to quote/cart |
PTH-93-20-03 | IMAC PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton | 96 | 827 USD | Add to quote/cart |
PTX-93-05-03 | IMAC PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix | 96 | 587 USD | Add to quote/cart |
PTX-93-05-03 | IMAC PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix | 96 | 587 USD | Add to quote/cart |
PTH-93-05-03 | IMAC PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton | 96 | 587 USD | Add to quote/cart |
PTT-95-16-03 | IMAC PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan | 96 | 2463 USD | Add to quote/cart |
PTT-95-20-03 | IMAC PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 827 USD | Add to quote/cart |
PTT-95-40-03 | IMAC PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan | 96 | 1168 USD | Add to quote/cart |
PTT-95-80-03 | IMAC PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan | 96 | 1851 USD | Add to quote/cart |
Biotage is a global supplier and solutions partner to a wide range of customers within drug discovery and development and analytical testing. With strong expertise in separation and purification technologies, and a focus on leveraging intelligent workflow solutions, we aim to efficiently support the advancement and protection of human health.
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