Parallel Peptide Purification

Rapid, environmentally friendly purification for peptide libraries

When synthesizing peptide libraries, the most time consuming aspect of the workflow is the purification step. HPLC, traditionally used for peptide purification, is relatively slow, so for large numbers of samples can become a serious bottleneck, as well as consuming large volumes of solvents. However, it is possible to leverage automated SPE (solid phase extraction) technologies specifically to allow the purification of peptides. Biotage have developed SPE technologies specifically for high-throughput peptide purification applications.

Peptide library purification the Biotage way

The Biotage approach to peptide library purification involves the use of automated solid phase extraction. By using a pre-packed 96 well plate, dedicated to peptide purification, Biotage® PeptiRen, and a fully automated plate processing system, the Biotage® Extrahera™, target peptide molecules from parallel synthesis workflows reactions are rapidly isolated with a focus on maximized recovery with a purification suitable for further assays. The Biotage approach is to employ SPE as it is extremely rapid and reproducible, much greener than traditional methods with vastly reduced solvent consumption reduced, and very straightforward and simple to use with intuitive touch screen software, a robust and reliable design and assisted method development.

Product groups

Biotage® Extrahera™ Classic for Peptides

Biotage® Extrahera™-Peptide is a compact and fully automated plate processing workstation for peptide library purification, designed to complement Biotage peptide purification plates. Robust and reliable, Extrahera sets the standard in parallel peptide purification.

Biotage® PeptiRen-96 C18 100 Å 500 mg Plates

96 well plates specifically designed for the purification of peptides synthesized using parallel methodologies. Rapid and reliable high throughput purification of peptide libraries with vastly reduced solvent consumption over traditional techniques.

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Resources

Biotage® Peptide Workflow Brochure

Biotage solutions for the peptide synthesis workflow of synthesis, purification and evaporation.

Biotage® Peptide Customer Case

Customer case using Biotage peptide workflow solutions.

Biotage® Peptide Customer Case 2

Second customer case using Biotage peptide solutions.