ISOLUTE® PLD+ Protein and Phospholipid Removal Plates and Columns provide a very effective but extremely simple sample clean-up for LC-MS/MS analysis. Requiring next to no method development, ISOLUTE PLD+ can be integrated quickly and easily into routine workflow, increasing productivity and reducing instrument downtime.
Part No. | Description | Pack Size | |
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918-0005-AG | ISOLUTE® PLD+ Columns, 50 mg/1 mL (Tabless) | 100 | Add to quote |
918-0050-P01 | ISOLUTE® PLD+ Protein and Phospholipid Removal Plate | 1 | Add to quote |
ISOLUTE® PLD+ plates and columns deliver cleaner extracts than other phospholipid removal products, meaning lower detection limits can be achieved. In the example below, signal-to-noise when using ISOLUTE® PLD+ was more than double when compared with competitor products (figure 1, below), leading to an immediate enhancement in sensitivity.
Figure 1. Relative peak intensity, 20 pg/mL amitriptyline in plasma (Blue = ISOLUTE® PLD+, Orange = Competitor O, Red= Competitor P).
More than 99 % of phospholipids are removed as the sample passes through the multifunctional sorbent bed, leaving clean, purified extracts for analysis (figure 2). Removing phospholipids also improves analytical column lifetime, preventing the build-up of phospholipids over multiple injections, and maintaining analyte sensitivity over extended analytical runs.
Figure 2. Phospholipids in human plasma treated by a) protein precipitation and b) ISOLUTE® PLD+. Lysophospholipids and phospholipids present in plasma treated by protein precipitation (red and green respectively) are shown in 1b on an expanded scale for direct comparison with residual levels of lysophospholipids and phospholipids present in ISOLUTE® PLD+ treated plasma (purple and blue respectively).
Using a simple solvent crash/filtration based procedure, proteins and phospholipids are simultaneously removed from plasma and other blood based samples while high, reproducible analyte recoveries are maintained. The optimised frit arrangement acts as a depth filter, efficiently trapping precipitated proteins, without blocking or plugging.
ISOLUTE® PLD+ plates and 'tabless' columns have excellent flow characteristics, and do not plug or block during use. They can be processed easily using either positive pressure or vacuum based systems, and are compatible with most automated liquid handlers.
Up to 96 x 1 mL volume tabless format columns can be processed on Biotage® Pressure+ 96, Extrahera and VacMaster-96 systems using specially designed racks. This cost effective option is ideal for applications with variable sample numbers or for method development.
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Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in Drug Discovery and Development, Analytical Testing and Water and Environmental Testing.
Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.
Our customers span a broad range of market segments including Pharmaceutical, Biotech, Contract Research and Contract Manufacturers as well as Clinical, Forensic and Academic laboratories in addition to organizations focused on Food safety, Clean water and Environmental sustainability.
Biotage is headquartered in Uppsala in Sweden and employs approx. 500 people worldwide. The Group had sales of SEK 1,101 million in 2019 and our products are sold in more than 70 countries. Biotage share (BIOT) is listed in the Mid Cap segment on the NASDAQ Stockholm.
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