Plasmid Purification

Automated Purification of Transfection-grade Plasmid DNA by our Unique PhyTip® Column Technology

Highly pure plasmid DNA samples are essential for transfection, in vivo studies, cloning, sequencing, genome editing and other molecular biology assays. Biotage offers innovative solutions for automated purification of plasmid DNA from cell pellets for scientists working in the biopharmaceutical industry and biotechnology. We have used our patented PhyTip^® columns for protein purification applications since 2004, but this gentle and efficient purification process works perfectly to automate plasmid purification too! The result is highly pure, supercoiled plasmid DNA that is optimal for downstream assays.

plasmid DNA circle

PHR-96-000-10y

Proven Technology

A PhyTip column is a separation column packed in a pipette tip that processes the sample by a process called dual flow chromatography. This technology enable automation of purification protocols and also comes with some unique advantages such as high reproducibility and high purity of samples.

Product Groups

LysaDirect tips on 96 head

MiniPrep PhyTip® Columns

Purification of 15 μg plasmid DNA from 1 mL cell cultures.

phyprep001-1

Biotage® PhyPrep Maxi Mega Giga

Automated endotoxin-free plasmid purification.

News from our blog

Automation of MiniPrep in a Drug Discovery Setting

An automated workflow for MiniPrep plasmid purification in a drug discovery setting.

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Resources

Application Note

MiniPrep PhyTip Columns for Plasmid DNA Purification

Other Blocks in the Workflow

Turnkey automation systems for sample processing by PhyTip^® columns.

Automation Systems

About Biotage

Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in drug discovery and development, analytical testing and water and environmental testing.

Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.

Our customers span a broad range of market segments including pharmaceutical, biotech, contract research and contract manufacturers as well as clinical, forensic and academic laboratories in addition to organizations focused on food safety, clean water and environmental sustainability.

Biotage is headquartered in Uppsala in Sweden and employs more than 500 people worldwide. The Group had sales of 1,092 MSEK in 2020 and our products are sold in more than 70 countries. Biotage’s share (BIOT) is listed in the Mid Cap segment on the NASDAQ Stockholm.

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Purification of transfection-grade sequencing ready plasmid DNA.