Biotage offers innovative solutions for automated purification of plasmid DNA from cell pellets, for scientists working in the biopharmaceutical industry and biotechnology. We have used our patented dual flow chromatography technique for protein purification applications since 2004, but this gentle and efficient purification process works perfectly to automate plasmid purification too! The result is highly pure, supercoiled plasmid DNA that is optimal for downstream assays.
PhyPrep is the first automated solution for Maxi, Mega and Giga scale plasmid purification. Designed to deliver high quality, endotoxin free hypercoiled DNA ready for immediate transfection. Read more about the system here.
A PhyTip column is a separation column packed in a pipette tip, that processes the sample by a technique called dual flow chromatography. This technology enable automation of purification protocols and also comes with some unique advantages such as high reproducibility and high purity of samples.
Automated plasmid purification in miniprep scale offers consistent yields of up to 15 μg plasmid DNA per sample, from a 1 mL culture pellet. The highly pure plasmid DNA is suitable for transfection, transformation, sequencing, PCR and cloning. Our MiniPrep PhyTip columns are available for Beckman, Tecan, Hamilton and MEA 2 systems. REad more about this technology in our white paper.