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Antibody Purification
ProPlus PhyTip® Columns

ProPlus PhyTip® Columns

Automated Purification of IgG Antibodies

Our ProPlus and ProPlus LX PhyTip columns are used for effective automated purification of IgG antibodies. ProPlus (MabSelect Sure™, Cytiva) and ProPlus LX (MabSelect™ SuRe™ LX, Cytiva) are affinity medias with an engineered version of the Protein A affinity ligand that demonstrate attributes of both Protein A and Protein G. The MabSelect™ SuRe™ medias favor an oriented binding of IgGs for high capacity binding of IgGs from low concentration samples and increased selectivity to mouse IgGs. In some cases, MabSelect™ SuRe™ demonstrates a 3-5 fold greater recovery compared to Protein G.

MabSelect™ SuRe™ is a licensed trademark of Cytiva.

Accessories & Consumables

Benefits

PhyNexus, now part of Biotage, developed the PhyTip^®column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step.

ProPlus PhyTip® Columns - Product Specification Sheet
ProPlus PhyTip® Affinity Columns - The Antibody Purification System that is the Best of Protein A and Protein G

Read more about dual flow chromatography here.

70 - 90 % Recovery of IgGs

Due to maximization of binding of the sample, less protein is lost during the purification process. The result is very high sample recoveries.

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Sample Concentration and Purification in One

Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.

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IgG Purification in 15 min

Effective purification by PhyTip columns enables a full purification protocol of 96 samples in as little as 15 minutes.

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Validated Protocols

Our Protein A PhyTip columns comes with dual flow chromatography protocols that have been validated with real protein samples and can be transferred to any automated liquid handling system.

Learn more

Literature & Videos

PRODUCT PRESENTATION AND SPECIFICATION

ProPlus PhyTip® Columns

PRODUCT FLYER

ProPlus PhyTip® Columns

BROCHURE

Biomolecule Purification with PhyTip® Columns

APPLICATION NOTE

ProPlus PhyTip® Affinity Columns - The Purification Systems that is the Best of Protein A and Protein G

PDF

PhyTip® Columns Compatibility with Automation Systems Overview

SAFETY DATA SHEET

ProPlus PhyTip Column

SAFETY DATA SHEET

5x Capture Buffer (A) for Protein A, Protein G, ProPlus and ProPlus LX PhyTip Columns

SAFETY DATA SHEET

Wash Buffer (P) for Protein A, Protein G, ProPlus and ProPlus LX PhyTip Columns

SAFETY DATA SHEET

Elution Buffer (C) for Protein A, Protein G, ProPlus and ProPlus LX PhyTip Columns

SAFETY DATA SHEET

Neutralization Buffer (G) for Protein A, Protein G, ProPlus and ProPlus LX PhyTip Columns

Videos

PRODUCT VIDEO

Dual Flow Chromatography

This is how dual flow chromatography works - capture, wash, elute.

PRODUCT VIDEO

PhyTip Column Demonstration

Sample processing by PhyTip columns.

Part Numbers

When choosing a PhyTip® column for your purification process, the optimal resin bed volume of the column depends on the total amount of protein present in your samples. If you are working with very low protein concentrations of 10 μg/mL or lower, we recommend using a resin bed volume one or two sizes bigger than indicated below for an effective purification process.

We offer PhyTip® columns available for all major liquid handling systems. Download here our guide over PhyTip® column compatibility with different liquid handling systems.

If you are not sure which PhyTip® column will that work best in your protocol, our specialists are here to help you.

Protein Amount Per Sample (Sample Concentrations >10 ug/mL)

<50 µg
50 - 100 µg
100 - 200 µg
200 - 400 µg
400 - 800 µg
800 - 1600 µg
1600 - 3200 µg

Automation System

MEA 2, ME
Hamilton
Beckman iSeries
Tecan

Agilent Bravo
Beckman FX & NX
Dynamic Devices Lynx
Dynamic Devices Oasis

Opentrons
Perkin Elmer SciClone, Calipher Zephyr, MiniTrack, Janus MTD
Janus Varispan, Cybio Felix

Part No.	Description	Pack Size	List Price
PTB-91-10-26	ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTX-91-10-26	ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTH-91-10-26	ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTR-91-10-26	ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA 2. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTT-91-10-26	ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTB-91-16-26	ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTX-91-16-26	ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTH-91-16-26	ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTR-91-16-26	ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA 2. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTT-91-16-26	ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTB-91-20-26	ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTX-91-20-26	ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTH-91-20-26	ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTR-91-20-26	ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTT-91-20-26	ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTR-91-32-26	ProPlus LX PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA 2. Incl. buffer kit.	96	3337 USD	Add to quote/cart
PTB-91-40-26	ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Beckman. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTX-91-40-26	ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTH-91-40-26	ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTH-91-40-26	ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTR-91-40-26	ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA 2. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTT-91-40-26	ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTB-91-80-26	ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTX-91-80-26	ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTH-91-80-26	ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTR-91-80-26	ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA 2. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTT-91-80-26	ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTV-92-20-26	ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTB-92-20-26	ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTB-92-20-26	ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTR-92-20-26	ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTT-92-20-26	ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTB-92-05-26	ProPlus LX PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTR-92-05-26	ProPlus LX PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTT-92-05-26	ProPlus LX PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTX-93-20-26	ProPlus LX PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTH-93-20-26	ProPlus LX PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTX-93-05-26	ProPlus LX PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTH-93-05-26	ProPlus LX PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTT-95-16-26	ProPlus LX PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTT-95-20-26	ProPlus LX PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTT-95-40-26	ProPlus LX PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTT-95-80-26	ProPlus LX PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTB-91-10-07	ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTX-91-10-07	ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTH-91-10-07	ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTR-91-10-07	ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA 2. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTT-91-10-07	ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan. Incl. buffer kit.	96	711 USD	Add to quote/cart
PTB-91-16-07	ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTX-91-16-07	ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTH-91-16-07	ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTR-91-16-07	ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA 2. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTT-91-16-07	ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTB-91-20-07	ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTX-91-20-07	ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTH-91-20-07	ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTR-91-20-07	ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA 2	96	840 USD	Add to quote/cart
PTT-91-20-07	ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTR-91-32-07	ProPlus PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA 2. Incl. buffer kit.	96	3337 USD	Add to quote/cart
PTB-91-40-07	ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Beckman. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTX-91-40-07	ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTH-91-40-07	ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTR-91-40-07	ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA 2. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTT-91-40-07	ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTB-91-80-07	ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTX-91-80-07	ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTH-91-80-07	ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTR-91-80-07	ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA 2. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTT-91-80-07	ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan. Incl. buffer kit.	96	1932 USD	Add to quote/cart
PTV-92-20-07	ProPlus PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTB-92-20-07	ProPlus PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTR-92-20-07	ProPlus PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTT-92-20-07	ProPlus PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTB-92-05-07	ProPlus PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTR-92-05-07	ProPlus PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTT-92-05-07	ProPlus PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTX-93-20-07	ProPlus PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTH-93-20-07	ProPlus PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTX-93-05-07	ProPlus PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTH-93-05-07	ProPlus PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton. Incl. buffer kit.	96	625 USD	Add to quote/cart
PTT-95-16-07	ProPlus PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan. Incl. buffer kit.	96	2745 USD	Add to quote/cart
PTT-95-20-07	ProPlus PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan. Incl. buffer kit.	96	840 USD	Add to quote/cart
PTT-95-40-07	ProPlus PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan. Incl. buffer kit.	96	1221 USD	Add to quote/cart
PTT-95-80-07	ProPlus PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan. Incl. buffer kit.	96	1932 USD	Add to quote/cart

ProPlus PhyTip® Columns

Automated Purification of IgG Antibodies

Benefits

70 - 90 % Recovery of IgGs

Sample Concentration and Purification in One

IgG Purification in 15 min

Validated Protocols

Literature & Videos

Videos

Dual Flow Chromatography

PhyTip Column Demonstration

Part Numbers

About Biotage

Ask a specialist

ProPlus PhyTip® Columns

Automated Purification of IgG Antibodies

Benefits

70 - 90 % Recovery of IgGs

Sample Concentration and Purification in One

IgG Purification in 15 min

Validated Protocols

Literature & Videos

Videos

Dual Flow Chromatography

PhyTip Column Demonstration

Part Numbers

Your Cart

Related Products

Protein A PhyTip® Columns

Protein G PhyTip® Columns

IMAC PhyTip® Columns

MEA 2 System

About Biotage

Ask a specialist