Purification of Tagged Proteins

PhyTip® Columns for Automated Purification of Tagged Proteins

Recombinant proteins can be expressed carrying an affinity tag that can be used for affinity purification. The choice of affinity tag can influence the protein solubility, stability and biological activity. Our unique PhyTip columns enable automated purification of proteins with high recoveries, while being gentle on the protein and yield consistent results Read more about our technology here .

Our PhyTip columns are available packed with carefully selected high quality affinity medias for effective purification of tagged recombinant proteins with purification protocols that have been validated with real protein samples at customer sites all over the world.

BIOMARK2.128 - Recombinant Proteins Card Image1

PHR-96-000-10ab

Affinity Purification by PhyTip Columns

Our PhyTip columns are available with carefully selected affinity medias for purification of tagged proteins. We offer all our PhyTip columns for protein purification in resin bed volumes from 5 μL to 320 μL for purification of µg to mg protein samples. Sample volumes can be varied between 100 µL and 50 mL.

We offer pre-programmed automation systems for protein purification. However, our PhyTip columns are available for all major liquid handling systems – which allows you to use them on existing instrumentation you may already have in your lab.

Product Groups

biomark002.405 - IMAC

IMAC PhyTip® Columns

Purification of histidine-tagged proteins by high-capacity Ni-IMAC affinity media.

biomark002.407 - Streptavidin

Streptavidin PhyTip® Columns

Purification of biotin-tagged proteins by Streptavidin affinity media.

biomark002.406 - Glutathione

Glutathione PhyTip® Columns

Purification of GST-tagged proteins by Glutathione affinity media.

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Learn more about high-throughput protein purification strategies!

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Resources

Overview of Compatible Liquid Handling Systems

We offer PhyTip columns for all major liquid handling systems.

All resources

Other Blocks in the Workflow

Automated antibody purification by PhyTip columns packed with affinity medias.

Antibody Purification

Automated protein purification by ion exchange PhyTip columns.

Ion Exchange

Automated high throughput desalting and buffer exchange.

Gel Filtration PhyTip Columns

About Biotage

Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in drug discovery and development and diagnostics, analytical testing and water and environmental testing.

Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.

Our customers span a broad range of market segments including pharmaceutical, biotech, diagnostic, contract research and contract manufacturers as well as clinical, forensic and academic laboratories in addition to organizations focused on food safety, clean water and environmental sustainability.

Biotage is headquartered in Uppsala in Sweden and employs appr. 700 people worldwide. The Group had sales of 1,566 MSEK in 2022 and our products are sold in more than 80 countries. Biotage’s share (BIOT) is listed in the Large Cap segment on the NASDAQ Stockholm.

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Purification of tagged protein