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ISOLUTE® PE-AX Catch & Release and Scavenging of Acidic Compounds
ISOLUTE® PE-AX is a silica-based sorbent with a chemically bonded quaternary amine functional group. It is used for the isolation of a wide range of acidic compounds using both catch & release and scavenging protocols. ISOLUTE PE-AX is available packed into SPE cartridges for flow-through. Using the catch & release approach, acidic target compounds can be isolated from a range of reaction mixtures. ISOLUTE PE-AX cartridges can be used in a scavenging mode to remove excess acidic reagents, allowing neutral or basic compounds to pass straight through to the collection vessel.
During catch & release the media bed "catches” the compound of interest. Rinsing the cartridge with solvent removes impurities, leaving the compound of interest on the cartridge for subsequent elution by an appropriate solvent. Biotage has a range of cartridges depending on whether the product to be recovered is acidic, basic or neutral. Applications include: purification, solvent switch, concentrating & capturing and HPLC fractions.
1. Cartridge equilibration (can be optional)
2. Sample loading
3. Cartridge washing (impurity elution, product is retained)
4. Product elution
It may also be possible to retain impurities in step 3, such that the cartridge becomes a one pass, one step purification system.
Further Notes: equilibration, loading and elution solvents may be substituted. Base cartridge loading calculations on approx. 60% cartridge capacity for initial run, depending
on the product, loading may be increased further following optimization.
