ISOLUTE® 103 is a hydroxylated polystyrene divinylbenzene co-polymer. The resin is used for the isolation of a wide range of compounds from prep LC fractions using catch & release protocol. The approach replaces the need for time-consuming evaporation of aqueous based fractions.
During catch & release the media bed "catches” the compound of interest. Rinsing the cartridge with solvent removes impurities, leaving the compound of interest on the cartridge for subsequent elution by an appropriate solvent. Biotage has a range of cartridges depending on whether the product to be recovered is acidic, basic or neutral. Applications include: purification, solvent switch, concentrating & capturing and HPLC fractions.
Overall Process:
It may also be possible to retain impurities in step 3, such that the cartridge becomes a one pass, one step purification system.
1. Choose ISOLUTE 103 column, 5 times the total sample mass
2.Perform column equilibration, at 10–50 mL/min
3. Dilute sample to max 30% organic content
4. Load onto ISOLUTE 103 column, allow to flow under gravity
5. Wash column
6. Release organic product using methanol
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
103-0200-E | ISOLUTE® 103 2 g/25 mL | 20 | 669 USD | Add to quote/cart |
103-0020-BG | ISOLUTE® 103 200 mg/3 mL (Tabless) | 50 | 301 USD | Add to quote/cart |
103-0020-C | ISOLUTE® 103 200 mg/6 mL | 30 | 160 USD | Add to quote/cart |
103-0020-CG | ISOLUTE® 103 200 mg/6 mL (Tabless) | 30 | 175 USD | Add to quote/cart |
103-0500-E | ISOLUTE® 103 5 g/25 mL | 20 | 1610 USD | Add to quote/cart |
103-0050-C | ISOLUTE® 103 500 mg/6 mL | 30 | 307 USD | Add to quote/cart |
103-0050-CG | ISOLUTE® 103 500 mg/6 mL (Tabless) | 30 | 320 USD | Add to quote/cart |
Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in drug discovery and development and diagnostics, analytical testing and water and environmental testing.
Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.
Our customers span a broad range of market segments including pharmaceutical, biotech, diagnostic, contract research and contract manufacturers as well as clinical, forensic and academic laboratories in addition to organizations focused on food safety, clean water and environmental sustainability.
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