Glutathione PhyTip columns are used for automated purification of GST-tagged antibodies and other recombinant proteins. The affinity resin used in these columns demonstrate high binding affinity and capacity for affinity purification of functional, recombinant GST-tagged proteins. The GST-tag (glutathione S-transferase) is a small (26 kDa) protein that is known to improve the solubility of the expressed recombinant protein.
Our Glutathione PhyTip columns comes with validated DFC protocols for purification of GST-tagged proteins that can be transferred to any automated liquid handling system. Our buffer kits contain all the buffers is needed for the purification protocol. Note that the proper use of Glutathione PhyTip columns require slow flow rates of 0.06 mL/min.
PhyNexus now part of Biotage developed the PhyTip column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step.